PXD039451
PXD039451 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximity biotinylation of NCI-H226 cells by EGFR-FabID |
Description | NCI-H226 cells were pretreated with DMSO or gefitinib and unstimulated or stimulated with EGF. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis. |
HostingRepository | jPOST |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-12-25_04:35:29.436.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hidetaka Kosako |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; Acetyl; N6-biotinyl-L-lysine; L-methionine sulfoxide |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-01-15 00:22:48 | ID requested | |
1 | 2023-11-13 20:10:20 | announced | |
⏵ 2 | 2023-12-25 04:35:30 | announced | 2023-12-25: Updated PubMed. |
Publication List
Yamada K, Shioya R, Nishino K, Furihata H, Hijikata A, Kaneko MK, Kato Y, Shirai T, Kosako H, Sawasaki T, Proximity extracellular protein-protein interaction analysis of EGFR using AirID-conjugated fragment of antigen binding. Nat Commun, 14(1):8301(2023) [pubmed] |
Keyword List
submitter keyword: proximity biotinylation, NCI-H226, FabID, EGFR, gefitinib |
Contact List
Hidetaka Kosako | |
---|---|
lab head | |
Hidetaka Kosako | |
contact affiliation | Tokushima University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001990/ |