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PXD039449

PXD039449 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProximity biotinylation of Expi293F cells overexpressing EGFR by EGFR-FabID
DescriptionExpi293F cells were transfected with pcDNA3.1-EGFR and treated with AGIA-FabID or EGFR-FabID in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis.
HostingRepositoryjPOST
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-12-25_04:35:51.319.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHidetaka Kosako
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; Acetyl; N6-biotinyl-L-lysine; L-methionine sulfoxide
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-14 23:35:54ID requested
12023-11-13 19:44:12announced
22023-12-25 04:35:52announced2023-12-25: Updated PubMed.
Publication List
Yamada K, Shioya R, Nishino K, Furihata H, Hijikata A, Kaneko MK, Kato Y, Shirai T, Kosako H, Sawasaki T, Proximity extracellular protein-protein interaction analysis of EGFR using AirID-conjugated fragment of antigen binding. Nat Commun, 14(1):8301(2023) [pubmed]
Keyword List
submitter keyword: proximity biotinylation, Expi293F, FabID, EGFR
Contact List
Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliationTokushima University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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