PXD039448

PXD039448 is an original dataset announced via ProteomeXchange.

Title	Environment Modulates Protein Heterogeneity Through Transcriptional and Translational Stop Codon Miscoding
Description	Stop codon miscoding events give rise to longer proteins, which may alter the proteins function and thereby generate short-lasting phenotypic variability from a single gene. In order to systematically assess the frequency and origin of miscoding events, we designed a library of reporters. We introduced premature stop codons into mScarlet that enabled high-throughput quantification of protein synthesis termination errors in E.coli using fluorescent microscopy. We found that under stress conditions, stop codon miscoding may occur as high as 80 percent of the time, depending on the genetic context, suggesting that evolution frequently samples stop codon miscoding events. Targeted mass spectrometry and RNA-seq analyses showed that not only translational but also transcriptional errors contribute to stop codon miscoding. The RNA polymerase is more likely to misincorporate a nucleotide at premature stop codons. Proteome-wide mass -spectrometry revealed that temperature regulates the expression of cryptic peptides generated by stop codon miscoding in E.coli. Overall, our findings suggest that the environment influences the accuracy of protein production which increases protein heterogeneity when the organisms need to adapt to new conditions.
HostingRepository	MassIVE
AnnounceDate	2024-03-27
AnnouncementXML	Submission_2024-03-27_10:28:06.974.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tobias Jumel
SpeciesList	scientific name: Escherichia coli; common name: E. coli; NCBI TaxID: 562;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; Deamidated; Acetyl; S-carboxamidoethyl-L-cysteine; L-cysteic acid (L-cysteine sulfonic acid)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-14 04:57:29	ID requested
⏵ 1	2024-03-27 10:28:07	announced

no publication

submitter keyword: phenotypic mutations, stop codon readthrough, RNA polymerase error

Agnes Toth-Petroczy
contact affiliation	MPI-CBG
contact email	tothpet@mpi-cbg.de
lab head
Tobias Jumel
contact affiliation	MPI-CBG
contact email	tobias.jumel@web.de
dataset submitter

MassIVE dataset URI

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