PXD039446

PXD039446 is an original dataset announced via ProteomeXchange.

Title	CedA: A General Transcription Factor from E. coli with a Distinct Mechanism of Action
Description	Gene expression in E. coli is controlled by well-established mechanisms that activate or repress transcription. Here, we identify CedA as an unconventional transcription factor specifically associated with the RNA polymerase (RNAP) σ70 holoenzyme. Structural and biochemical analysis of CedA bound to RNAP reveal that it bridges distant domains of β and σ70 subunits to stabilize an open-promoter complex. Remarkably, CedA does so without contacting DNA. We further show that cedA is strongly induced in response to amino acid starvation, oxidative stress, and aminoglycosides. CedA provides a basal level of tolerance to these clinically relevant antibiotics, as well as to rifampicin and peroxide. Finally, we show that CedA modulates transcription of hundreds of bacterial genes, which explains its pleotropic effect on cell physiology and pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2023-09-17
AnnouncementXML	Submission_2023-09-17_07:43:53.100.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	NikitaVasilyev
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-13 16:11:57	ID requested
⏵ 1	2023-09-17 07:43:53	announced

Dataset with its publication pending

submitter keyword: transcription, initiation factor,RNA polymerase, antibiotic resistance

EvgenyNudler
contact affiliation	Department of Biochemistry and Molecular Pharmacology, NYU Grossman School of Medicine, New York, NY, US
contact email	evgeny.nudler@nyulangone.org
lab head
NikitaVasilyev
contact affiliation	NYU School of Medicine
contact email	nikita.vasilyev@nyulangone.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD039446

PRIDE project URI

• PRIDE
  1. PXD039446
     1. Label: PRIDE project
     2. Name: CedA: A General Transcription Factor from E. coli with a Distinct Mechanism of Action