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PXD039416

PXD039416 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEx vivo proteomics of Mycobacterium marinum
DescriptionProteins expressed and exported out of the mycobacterial biofilm cells into granulomatous environment during infection were identified by LC-MS/MS. To this end, adult 5- to 10-month-old female AB wild-type zebrafish (Danio rerio) were infected with 4-day old M. marinum ATCC927 (carrying the pTEC27 plasmid expressing the tdTomato fluorescent protein) cells. After 8 weeks post infection, ten zebrafish were euthanized, and red fluorescent mycobacterial granulomas were carefully dissected from the zebrafish ovaries. Ten granulomas were collected per tube, frozen on dry ice and stored at -80 °C until preparation for proteomics. Altogether, ten replicate samples, each with ten mycobacterial granulomas, were subjected to protein extraction and LC-MS/MS identification.
HostingRepositoryPRIDE
AnnounceDate2023-03-06
AnnouncementXMLSubmission_2023-03-06_03:47:23.283.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTuulaNyman
SpeciesList scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF fleX
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-13 06:49:17ID requested
12023-03-06 03:47:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PACM sample preparation, MaxQuant, LC-MS/MS identification, mycobacterial surface and secreted proteins
Contact List
TuulaNyman
contact affiliationHead of Proteomics
contact emailtuula.nyman@medisin.uio.no
lab head
TuulaNyman
contact affiliationUniversity of Oslo
contact emailtuula.nyman@medisin.uio.no
dataset submitter
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Dataset FTP location
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PRIDE project URI
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