PXD039401

PXD039401 is an original dataset announced via ProteomeXchange.

Title	Identification of the E3 ligase(s) recruited by 955 to degrade CDK9
Description	PROTACs induce the degradation of target proteins by hijacking an E3 ligase. However, it is usually difficult to directly identify the E3 ligase recruited by a new E3 ligase ligand in a PROTAC using the co-immunoprecipitation method because the formation of the complex is transient and very dynamic. TurboID assay is a powerful proximity labeling method that can sensitively detect weak and transient protein-protein interactions by biotinylating proteins that interact with a bait protein fused with an engineered biotin ligase. Here, we adapted TurboID technology to identify and characterize the specific E3 ligase(s) recruited by 955, a potent piperlongumine (PL)-SNS-032 conjugated CDK9 PROTAC, to mediate CDK9 degradation.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_01:04:06.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongwen Lyu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-13 06:04:08	ID requested
⏵ 1	2024-05-24 01:04:07	announced

Pei J, Xiao Y, Liu X, Hu W, Sobh A, Yuan Y, Zhou S, Hua N, Mackintosh SG, Zhang X, Basso KB, Kamat M, Yang Q, Licht JD, Zheng G, Zhou D, Lv D, Piperlongumine conjugates induce targeted protein degradation. Cell Chem Biol, 30(2):203-213.e17(2023) [pubmed]

10.1016/j.chembiol.2023.01.004;

submitter keyword: TurboID, PROTAC,PL, CDK9, E3 ligase

Dongwen Lv
contact affiliation	Department of Biochemistry and Structural Biology and Center for Innovative Drug Discovery School of Medicine, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, United States
contact email	lyud@uthscsa.edu
lab head
Dongwen Lyu
contact affiliation	UT Health San Antonio
contact email	lyud@uthscsa.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD039401

PRIDE project URI

• PRIDE
  1. PXD039401
     1. Label: PRIDE project
     2. Name: Identification of the E3 ligase(s) recruited by 955 to degrade CDK9