PXD039400

PXD039400 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming
Description	Sexual reproduction of Toxoplasma gondii, which is restricted to the small intestine of felids, is sparsely documented in studies involving domestic cats, which also raises ethical concerns. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described. Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites, as revealed by mass spectrometry-based proteomic analyses of the parasites.
HostingRepository	PRIDE
AnnounceDate	2024-01-29
AnnouncementXML	Submission_2024-01-29_06:53:14.972.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039400
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-13 06:03:53	ID requested
1	2023-09-16 07:19:49	announced
⏵ 2	2024-01-29 06:53:15	announced	2024-01-29: Updated project metadata.

Publication List

10.6019/PXD039400;
10.1038/s41586-023-06821-y;
Antunes AV, Shahinas M, Swale C, Farhat DC, Ramakrishnan C, Bruley C, Cannella D, Robert MG, Corrao C, Cout, é Y, Hehl AB, Bougdour A, Coppens I, Hakimi MA, In vitro production of cat-restricted Toxoplasma pre-sexual stages. Nature, 625(7994):366-376(2024) [pubmed]

10.6019/PXD039400;

10.1038/s41586-023-06821-y;

Antunes AV, Shahinas M, Swale C, Farhat DC, Ramakrishnan C, Bruley C, Cannella D, Robert MG, Corrao C, Cout, é Y, Hehl AB, Bougdour A, Coppens I, Hakimi MA, In vitro production of cat-restricted Toxoplasma pre-sexual stages. Nature, 625(7994):366-376(2024) [pubmed]

Keyword List

submitter keyword: merogony,Toxoplasma gondii, sexual commitment, Apetala, transcription factor, gene expression, nanoLC-MS/MS, epigenetic, chromatin

submitter keyword: merogony,Toxoplasma gondii, sexual commitment, Apetala, transcription factor, gene expression, nanoLC-MS/MS, epigenetic, chromatin

Contact List

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD039400
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD039400

PRIDE project URI

Repository Record List

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