PXD039394

PXD039394 is an original dataset announced via ProteomeXchange.

Title	IP-MS:Protein precipitated by antisense (AS) DNA oligomers crossing the junction site of circNEIL3
Description	To identify the circNEIL3 interacting protein, we performed RNA pull-down assays after transfecting into cells with biotin-labeled sense (S) or antisense (AS) DNA oligomers crossing the junction site of circNEIL3, and the pull-down proteins were subjected to SDS-PAGE and Coomassie blue staining. ~45 kDa Band pulled down by the AS probe was specifically excised , digested and used for mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:44.011.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuang Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-13 05:49:28	ID requested
1	2023-10-24 11:32:43	announced
⏵ 2	2023-11-14 09:07:46	announced	2023-11-14: Updated project metadata.

Chen S, Li K, Guo J, Chen HN, Ming Y, Jin Y, Xu F, Zhang T, Yang Y, Ye Z, Liu W, Ma H, Cheng J, Zhou JK, Li Z, Shen S, Dai L, Zhou ZG, Xu H, Peng Y, circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1. Proc Natl Acad Sci U S A, 120(13):e2215132120(2023) [pubmed]

submitter keyword: RNA-pulldown,Gel-based proteomics,MDA-MB-231 cell line

Yong Peng
contact affiliation	Laboratory of Molecular Oncology, Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
contact email	yongpeng@scu.edu.cn
lab head
Shuang Chen
contact affiliation	State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
contact email	316479854@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD039394

PRIDE project URI

• PRIDE
  1. PXD039394
     1. Label: PRIDE project
     2. Name: IP-MS:Protein precipitated by antisense (AS) DNA oligomers crossing the junction site of circNEIL3