PXD039388

PXD039388 is an original dataset announced via ProteomeXchange.

Title	Tankyrase-1regulates RBP-mediated mRNA turnover to promotemuscle fiber formation.
Description	Poly(ADP-ribosylation)(PARylation)is apost-translational modification mediated by asubset of ADP-ribosyl transferases (ARTs). Although PARylation-inhibition based therapies areconsidered as an avenue to combat debilitating diseases such as cancerand myopathies, the roleof thismodificationinphysiological processes such ascell differentiation remainsunclear. Here we showthat Tankyrase1 (TNKS1), aPARylating ART, plays a major role in myogenesis, a vital process known to drivemuscle fiber formation and regeneration.Althoughallbona fidePARPs are expressed in muscle cells, experiments using siRNA-mediated knockdownor pharmacological inhibitionshow thatTKNS1 is the enzyme responsible ofcatalyzing PARylation during myogenesis. Via this activity,TKNS1controlsthe turnoverof mRNAs encoding myogenic regulatory factors such as nucleophosmin(NPM) and myogenin.TKNS1 mediates these effectsby targeting RNA-binding proteinssuch as Human Antigen R (HuR). HuR harbors a conserved TNKS-binding motif (TBM), the mutation of which not only prevents theassociation of HuR with TKNS1 anditsPARylation, but also precludes HuR from regulating the turnover of NPMand myogeninmRNAsas well as from promoting myogenesis. Therefore, our data uncovera new role forTNKS1as akeymodulator of RBP-mediated post transcriptionaleventsrequired for vitalprocesses suchasmyogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_00:58:24.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brenda Janice Sanchez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-13 05:45:07	ID requested
⏵ 1	2024-05-24 00:58:25	announced

10.1093/nar/gkae059;

Mubaid S, Sanchez BJ, Algehani RA, Skopenkova V, Adjibade P, Hall DT, Busque S, Lian XJ, Ashour K, Tremblay AK, Carlile G, Gagn, é JP, Diaz-Gaxiola A, Khattak S, Di Marco S, Thomas DY, Poirier GG, Gallouzi IE, Tankyrase-1 regulates RBP-mediated mRNA turnover to promote muscle fiber formation. Nucleic Acids Res, 52(7):4002-4020(2024) [pubmed]

submitter keyword: PARylation, Tankyrase,Muscle, HuR, post-transcriptional regulation

Imed E. Gallouzi
contact affiliation	KAUST Smart-Health Initiative(KSHI)and Biological and Environmental Science and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Jeddah, Saudi Arabia. Dept. of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC, H3G1Y6, Canada. Rosalind & Morris Goodman Cancer Institute, McGill University, 1160 Pine Avenue, Montreal, QC, H3A1A3, Canada.
contact email	gallouzi.imed@kaust.edu.sa
lab head
Brenda Janice Sanchez
contact affiliation	KAUST
contact email	brendajanice.sanchez@kaust.edu.sa
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD039388

PRIDE project URI

• PRIDE
  1. PXD039388
     1. Label: PRIDE project
     2. Name: Tankyrase-1regulates RBP-mediated mRNA turnover to promotemuscle fiber formation.