PXD039381

PXD039381 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of sialoliths from calcified, lipid and mixed groups as a source of potential biomarkers of deposit formation in the salivary glands.
Description	Salivary stones, also known as sialoliths, are formed in a pathological situation in the salivary glands. So far, neither the mechanism of their formation nor the factors predisposing to their formation are known. Although they do not directly threaten human life, they significantly deteriorate the patient's quality of life. Although this is not a typical research material, attempts are made to apply various analytical tools to characterize sialoliths and search for their biomarkers in their proteins. In this work, we used mass spectrometry and SWATH-MS qualitative and quantitative analysis to investigate the composition and select proteins that may contribute to solid deposits in the salivary glands. Twenty sialoliths, previously characterized spectroscopically and divided into the following groups: calcified (CAL), lipid (LIP) and mixed (MIX), were used for the study. Proteins unique for each of the groups were found, including: for the CAL group among them, e.g. proteins from the S100 group (S100 A8/A12 and P), mucin 7 (MUC7), keratins (KR1/2/4/5/13), elastase (ELANE) or stomatin (STOM); proteins for the LIP group - transthyretin (TTR), lactotransferrin (LTF), matrix Gla protein (MPG), submandibular gland androgen-regulated protein 3 (SMR3A); mixed stones had the fewest unique proteins. Bacterial proteins present in sialoliths have also been identified. The analysis of the results indicates the possible role of bacterial infections, disturbances in calcium metabolism and neutrophil extracellular traps (NETs) in the formation of sialoliths.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:45.056.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalia Musiał
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-13 05:31:10	ID requested
1	2023-05-10 14:50:57	announced
⏵ 2	2023-11-14 08:47:56	announced	2023-11-14: Updated project metadata.

Musia, ł N, Bogucka A, Tretiakow D, Skorek A, Ryl J, Czaplewska P, Proteomic analysis of sialoliths from calcified, lipid and mixed groups as a source of potential biomarkers of deposit formation in the salivary glands. Clin Proteomics, 20(1):11(2023) [pubmed]

submitter keyword: functional analysis, mass spectrometry,sialolithiasis, salivary stones, SWATH-MS

Paulina Czaplewska
contact affiliation	Laboratory of Mass Spectrometry Intercollegiate Faculty of Biotechnology UG-MUG
contact email	paulina.czaplewska@ug.edu.pl
lab head
Natalia Musiał
contact affiliation	Intercollegiate Faculty of Biotechnology UG&MUG
contact email	natalia.musial@phdstud.ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039381

PRIDE project URI

• PRIDE
  1. PXD039381
     1. Label: PRIDE project
     2. Name: Proteomic analysis of sialoliths from calcified, lipid and mixed groups as a source of potential biomarkers of deposit formation in the salivary glands.