⮝ Full datasets listing

PXD039381

PXD039381 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of sialoliths from calcified, lipid and mixed groups as a source of potential biomarkers of deposit formation in the salivary glands.
DescriptionSalivary stones, also known as sialoliths, are formed in a pathological situation in the salivary glands. So far, neither the mechanism of their formation nor the factors predisposing to their formation are known. Although they do not directly threaten human life, they significantly deteriorate the patient's quality of life. Although this is not a typical research material, attempts are made to apply various analytical tools to characterize sialoliths and search for their biomarkers in their proteins. In this work, we used mass spectrometry and SWATH-MS qualitative and quantitative analysis to investigate the composition and select proteins that may contribute to solid deposits in the salivary glands. Twenty sialoliths, previously characterized spectroscopically and divided into the following groups: calcified (CAL), lipid (LIP) and mixed (MIX), were used for the study. Proteins unique for each of the groups were found, including: for the CAL group among them, e.g. proteins from the S100 group (S100 A8/A12 and P), mucin 7 (MUC7), keratins (KR1/2/4/5/13), elastase (ELANE) or stomatin (STOM); proteins for the LIP group - transthyretin (TTR), lactotransferrin (LTF), matrix Gla protein (MPG), submandibular gland androgen-regulated protein 3 (SMR3A); mixed stones had the fewest unique proteins. Bacterial proteins present in sialoliths have also been identified. The analysis of the results indicates the possible role of bacterial infections, disturbances in calcium metabolism and neutrophil extracellular traps (NETs) in the formation of sialoliths.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:47:45.056.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNatalia Musiał
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-13 05:31:10ID requested
12023-05-10 14:50:57announced
22023-11-14 08:47:56announced2023-11-14: Updated project metadata.
Publication List
Musia, ł N, Bogucka A, Tretiakow D, Skorek A, Ryl J, Czaplewska P, Proteomic analysis of sialoliths from calcified, lipid and mixed groups as a source of potential biomarkers of deposit formation in the salivary glands. Clin Proteomics, 20(1):11(2023) [pubmed]
Keyword List
submitter keyword: functional analysis, mass spectrometry,sialolithiasis, salivary stones, SWATH-MS
Contact List
Paulina Czaplewska
contact affiliationLaboratory of Mass Spectrometry Intercollegiate Faculty of Biotechnology UG-MUG
contact emailpaulina.czaplewska@ug.edu.pl
lab head
Natalia Musiał
contact affiliationIntercollegiate Faculty of Biotechnology UG&MUG
contact emailnatalia.musial@phdstud.ug.edu.pl
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039381
PRIDE project URI
Repository Record List
[ + ]