PXD039376

PXD039376 is an original dataset announced via ProteomeXchange.

Title	Proteome Profiling of Endogenous and Potential S-nitrolysation in Colorectal Cancer
Description	Background. As a common cancer with high incidence rate and mortality, colorectal cancer (CRC) is seriously threatening human health. Nitric oxide (NO) mediated protein S-nitrosylation (SNO) is of great significance in the occurrence and development of CRC, melanoma, breast cancer and other cancers. Materials and methods. In this study, the proteomic results of human cancer tissues, para-cancerous tissues, SW480, NCM460 and SW620, which were treated by biotin switch and magnetic beads enrichment technology, were analyzed by mass spectrometry (MS). Through the comparison and analysis of MS results and literatures, some proteins with potential significance were identified in CRC. Results. 105 peptides with SNO sites were screened from human tissues. 128 peptides with SNO sites were screened from SW480 and NCM460 cell lines. Through the cross validation of MS results and literatures, several SNO proteins were identified frequently in CRC, such as the nucleoside diphosphate kinase A (NDK A), desmocollin-1, protein S100-A8, Pyruvate kinase PKM, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), etc. Conclusion. We believed that NDK A, desmocollin-1, protein S100-A8, Pyruvate kinase PKM, GAPDH and other related proteins had important biological significance in CRC.
HostingRepository	iProX
AnnounceDate	2023-01-13
AnnouncementXML	Submission_2024-06-06_01:18:08.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feng Liang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-12 19:06:16	ID requested
1	2023-04-13 18:51:39	announced
⏵ 2	2024-06-06 01:18:09	announced	2024-06-06: Update information.

Liang F, Wang S, Guo Y, Mu Y, Shang F, Wang M, Proteome profiling of endogenous and potential S-nitrosylation in colorectal cancer. Front Endocrinol (Lausanne), 14():1153719(2023) [pubmed]

submitter keyword: Colorectal Cancer, S-nitrosylation, Proteomics, Biotin switch, S-Nitrosoglutathione.

Min Wang
contact affiliation	Second Hospital of Jilin University
contact email	wangmin2009@jlu.edu.cn
lab head
Feng Liang
contact affiliation	Second Hospital of Jilin University
contact email	1765367232@qq.com
dataset submitter

iProX dataset URI