PXD039371
PXD039371 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of Trityl Post-translational modification by an untargeted bottom-up proteomic-based approach using UHPLC-Q Exactive Hybrid Quadrupole-Orbitrap high resolution mass spectrometry |
| Description | We adopted an untargeted bottom-up proteomic-based approach with a high-resolution tandem-mass spectrometry for assessment of pesticide mixture induced neurotoxicity on human brain tumor meningiomas and in-depth elucidation of their involved mechanisms through protein-adduct formation or protein-Post translational modifications with prediction of alteration sites by de novo peptides sequencing. Three proteins namely, Glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), alpha-enolase (ENO1) and Phosphoglycerate-kinase-1 (PGK1) were selected as key targets in oxidative stress-response. Freshly resected human brain tumors were cultured in neural stem-cell culture medium for a 3D neurospheroid development model, then treated by pesticide mixture at relevant concentrations. Afterwards, proteins were extracted, reduced and alkylated, then digested by trypsin. The extracted peptides were analysed by a nano-UHPLC-ESI-Q Exactive HF hybrid quadrupole-Orbitrap high resolution mass spectrometry. Raw MS files were searched against human protein database based on the species of the samples using Maxquant (1.6.1.14). |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-11-13 |
| AnnouncementXML | Submission_2024-11-13_22:17:01.529.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | KAOUTHAR |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | triphenylmethyl |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-01-12 09:21:48 | ID requested | |
| ⏵ 1 | 2024-11-13 22:17:01 | announced |
Publication List
| Kaouthar Louati,* Amina Maalej, Fatma Kolsi, Rim Kallel, Yassine Gdoura, Mahdi Borni, Leila Sellami Hakim, Rania Zribi, Sirine Choura, Sami Sayadi, Mohamed Chamkha, Basma Mnif, Zouheir Khemakhem, Tahya Sellami Boudawara, Mohamed Zaher Boudawara, Abderrahman Bouraoui, Jamil Kraiem, and Fathi Safta. A shotgun proteomic-based approach with Q-Exactive Hybrid Quadrupole-Orbitrap High-Resolution mass spectrometer for assessment of pesticide mixture induced neurotoxicity on 3D-developed neurospheroid model from human brain meningiomas: Identification of trityl-post-translational modification. https://doi.org/10.1021/acs.jproteome.4c00804. |
Keyword List
| submitter keyword: Nanow-flow UPLC, tandem mass spectrometry (MS/MS), bottom-up proteomic-based approach, Human brain tumors, Neurospheroids, PTMs, triphenylmethyl, pesticides |
Contact List
| Kaouthar LOUATI | |
|---|---|
| contact affiliation | University of Monastir, Faculty of Pharmacy, Laboratory of Pharmacology, Analytics and Galenic Drug Development- LR12ES09, Road Avicenne 5000, Monastir, Tunisia |
| contact email | kaoutharlouati66@gmail.com |
| lab head | |
| KAOUTHAR | |
| contact affiliation | University of Monastir, Faculty of Pharmacy, |
| contact email | kaoutharlouati66@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091053/ |
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