PXD039357

PXD039357 is an original dataset announced via ProteomeXchange.

Title	The anti-Streptococcus agalactiae planktonic cells and biofilm activity of Ginkgolic Acid (15:1) and the main mechanism of bacteriostasis
Description	S. agalactiae NSGC54 culture in logarithmic growth phase was added GA (15:1) to a final concentration of 3.125μM and control group treated with dmso. RIPA lysis buffer was used to lyse the collected protein solution. The total protein concentration of the cell lysate was measured by the BCA kit (Beyotime Biotechnology, Shanghai, China). The cell lysate was incubated with dmso and GA (15:1) at 4 °C for 30 minutes, and the protease K was added at a mass ratio of 1:500 for digestion at room temperature for 30 minutes. The loading buffer was added to terminate the digestion and the water bath was heated at 70 °C for 10 minutes. Finally, differential proteins were identified by mass spectrometry and bioinformatics analysis.
HostingRepository	iProX
AnnounceDate	2023-01-11
AnnouncementXML	Submission_2023-01-10_19:54:30.323.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cong Wang
SpeciesList	scientific name: Streptococcus agalactiae STIR-CD-17; NCBI TaxID: 1097670;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-10 19:54:15	ID requested
⏵ 1	2023-01-10 19:54:30	announced

Dataset with its publication pending

submitter keyword: ginkgolic acid (15: 1), streptococcus agalactiae, drug affinity responsive target stability

Zewen Wen
contact affiliation	Huazhong University of Science and Technology Union Shenzhen Hospital
contact email	wenzw05@163.com
lab head
Cong Wang
contact affiliation	Huazhong University of Science and Technology Union Shenzhen Hospital
contact email	1403375061@qq.com
dataset submitter

iProX dataset URI