PXD039335

PXD039335 is an original dataset announced via ProteomeXchange.

Title	Structural and regulatory insights into the glideosome-associated connector from Toxoplasma gondii
Description	The phylum of Apicomplexa groups intracellular parasites that employ substrate-dependent gliding motility to invade host cells, egress from the infected cells and cross biological barriers. The glideosome associated connector (GAC) is a conserved protein essential to this process. GAC facilitates the association of actin filaments with surface transmembrane adhesins and the efficient transmission of the force generated by myosin translocation of actin to the cell surface substrate. Here, we present the crystal structure of Toxoplasma gondii GAC and reveal a unique, supercoiled armadillo repeat region that adopts a closed ring conformation. Characterisation of the membrane binding interface within the C-terminal PH domain as well as an N-terminal fragment necessary for association with F-actin suggest that GAC adopts multiple conformations. A multi-conformational model for assembly of GAC within the glideosome is proposed
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:11.550.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oscar Vadas
SpeciesList	scientific name: Toxoplasma gondii RH-88; NCBI TaxID: 1208370;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-09 02:33:14	ID requested
1	2023-05-10 13:56:42	announced
⏵ 2	2023-11-14 08:47:16	announced	2023-11-14: Updated project metadata.

Kumar A, Vadas O, Dos Santos Pacheco N, Zhang X, Chao K, Darvill N, Rasmussen H Ø, Xu Y, Lin GM, Stylianou FA, Pedersen JS, Rouse SL, Morgan ML, Soldati-Favre D, Matthews S, . Elife, 12():(2023) [pubmed]

ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)

submitter keyword: Apicomplexa, gliding motility, X-ray crystallography, HDX-MS, Toxoplasma gondii

Dominique Soldati-Favre
contact affiliation	University of Geneva, Department of Microbiology and Molecular Medicine
contact email	Dominique.Soldati-Favre@unige.ch
lab head
Oscar Vadas
contact affiliation	University of Geneva, Faculty of Medicine
contact email	oscar.vadas@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039335

PRIDE project URI

• PRIDE
  1. PXD039335
     1. Label: PRIDE project
     2. Name: Structural and regulatory insights into the glideosome-associated connector from Toxoplasma gondii