PXD039333

PXD039333 is an original dataset announced via ProteomeXchange.

Title	Mouse RNF138 immunoprecipitation mass spectrography analysis in macrophages
Description	As one of the core components of the SWI/SNF complex, SMARCC1 (BAF155, SRG3) plays essential roles in activation of the late inflammatory genes in response to microbial challenge. However, little is known about the mechanism how SMARCC1 regulates the inflammatory innate response. Via functional screening, we identify nuclear E3 ubiquitin ligase RNF138 as a negative regulator in inflammatory innate response, and show that RNF138 interacts with SMARCC1 and mediates its K48-linked polyubiquitination at position Lys643 and proteasomal degradation. As a result, catalytic activity of RNF138 fine-tunes the kinetics of late inflammatory gene transcription by inhibiting chromatin remodeling at SWI/SNF regulated gene loci. The reduced RNF138 and increased SMARCC1 in monocytes of rheumatoid arthritis patients are observed. These results provide mechanistic insight into the interplay among nucleosome remodeling, inflammation and ubiquitylation, and underscore the important role of the E3 ubiquitin ligases in controlling both extent and duration of inflammatory responses.
HostingRepository	PRIDE
AnnounceDate	2023-01-10
AnnouncementXML	Submission_2023-01-09_17:24:18.556.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiLiu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-09 02:32:24	ID requested
⏵ 1	2023-01-09 17:24:18	announced

Dataset with its publication pending

submitter keyword: mass spectrography analysis,mouse, macrophages, RNF138

MinghongJiang
contact affiliation	Chinese Academy of Medical Sciences
contact email	jiangminghong@163.com
lab head
WeiLiu
contact affiliation	Chinese Academy of Medical Sciences
contact email	nmgjnlw@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD039333

PRIDE project URI

• PRIDE
  1. PXD039333
     1. Label: PRIDE project
     2. Name: Mouse RNF138 immunoprecipitation mass spectrography analysis in macrophages