PXD039322

PXD039322 is an original dataset announced via ProteomeXchange.

Title	A CAPSID PROTEIN FRAGMENT OF A TOTI-LIKE VIRUS FOUND IN CARICA PAPAYA LATEX INTERACTS WITH THE 50S RIBOSOMAL PROTEIN L17
Description	Papaya sticky disease is caused by the association of a toti-like and an umbravirus-like associated RNA (ulaRNA), named papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), respectively. Both viral genomes are encapsidated in particles formed by PMeV ORF1 product, which has the potential to encode a protein with 1563 amino acids (aa). However, the exact composition of the viral capsid is unknown. To characterize the structural proteins of PMeV and PMeV2, virions were purified from Carica papaya latex. SDS-PAGE analysis of purified virus revealed two major proteins of ~40 kDa and ~55 kDa. Amino-terminal sequencing of the ~55 kDa protein and LC-MS/MS of purified virions indicated that this protein starts at aa 263 of the deduced ORF1 product as result of a degradation or proteolytic processing. A yeast two-hybrid assay was used to identify Arabidopsis proteins interacting with two PMeV ORF1 product fragments (aa 321-670 and 961-1200). The 50S ribosomal protein L17 (AtRPL17) was identified as potentially associated with modulated translation-related proteins. In plant cells, AtRPL17 co-localized and interacted with the PMeV ORF1 fragments. These findings support the hypothesis that the interaction between PMeV/PMeV2 structural proteins and RPL17 is important for virus-host interactions.
HostingRepository	PRIDE
AnnounceDate	2025-09-04
AnnouncementXML	Submission_2025-09-04_11:11:29.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Unit
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-09 02:11:43	ID requested
⏵ 1	2025-09-04 11:11:29	announced

10.3390/V15020541;

ProteomeXchange project tag: Food and Nutrition (B/D-HPP)

submitter keyword: Totiviridae

protein-protein interaction

coat protein

dsRNA

plant virus

Fábio César Sousa Nogueira
contact affiliation	Proteomics Unit, Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Av Athos da Silveira Ramos, 149, CT- A-542, Cidade Universitária 21941-909 Rio de Janeiro, RJ, Brazil
contact email	fabiocsn@gmail.com
lab head
Proteomics Unit
contact affiliation	UFRJ
contact email	unidadeproteomica@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039322
     1. Label: PRIDE project
     2. Name: A CAPSID PROTEIN FRAGMENT OF A TOTI-LIKE VIRUS FOUND IN CARICA PAPAYA LATEX INTERACTS WITH THE 50S RIBOSOMAL PROTEIN L17