To acquired insights into the pyoluteorin-mediated physiological process of P. fluorescens, label-free quantitative proteomics was used to compare the expression differences between the whole proteome of Pf-5 with and without pyoluteorin. To further investigate the importance of PhlG in physiological processes of P. protegens Pf-5, we performed a quantitative proteomic profiling of wide type and ΔphlG strain.