PXD039316

PXD039316 is an original dataset announced via ProteomeXchange.

Title	DeKinomics pulse-chases kinase functions in living cells
Description	Cellular context is crucial for understanding the complex and dynamic kinase functions in health and disease. Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation (“pulse”) of a kinase of interest, as well as global and in-depth characterization (“chase”) of the perturbed proteome under living conditions. We herein developed an optogenetic “pulse-chase” strategy, termed Decaging Kinase coupled proteomics (DeKinomics), for proteome-wide profiling of kinase-driven phosphorylation at second-timescale in living cells. We took advantage of the “gain-of-function” feature of DeKinomics to identify direct kinase substrates and further portrayed the global phosphorylation of understudied receptor tyrosine kinases (RTKs) under native cellular settings. DeKinomics offered a general activation-based strategy to study kinase functions with high specificity and temporal resolution under living conditions.
HostingRepository	iProX
AnnounceDate	2023-10-07
AnnouncementXML	Submission_2024-05-21_00:46:57.208.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yicheng Weng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue; deaminated residue; iodoacetamide derivatized residue; methionine oxidation with neutral loss of 64 Da
Instrument	Q Exactive HF-X; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-08 20:04:34	ID requested
1	2023-12-10 20:15:46	announced
⏵ 2	2024-05-21 00:46:58	announced	2024-05-21: Update information.

Weng Y, Chen W, Kong Q, Wang R, Zeng R, He A, Liu Y, Mao Y, Qin Y, Ngai WSC, Zhang H, Ke M, Wang J, Tian R, Chen PR, DeKinomics pulse-chases kinase functions in living cells. Nat Chem Biol, 20(5):615-623(2024) [pubmed]

submitter keyword: Phosphoproteomics, pTyr proteomics

Ruijun Tian
contact affiliation	Southern University of Science and Technology
contact email	tianrj@sustech.edu.cn
lab head
Yicheng Weng
contact affiliation	Southern University of Science and Technology
contact email	wengyc12@qq.com
dataset submitter

iProX dataset URI