PXD039314

PXD039314 is an original dataset announced via ProteomeXchange.

Title	Proteomics of obstructed kidneys from patients with obstructive nephropathy and unilateral ureteral obstruction mice
Description	Here, we performed proteomics of kidneys obtained from patients with obstructive nephropathy and healthy controls (n=11/8), or from unilateral ureteral obstruction (UUO) mice and sham mice (n=6/6). Proteomic measurement of all human kidney samples resulted in a total of 6089 proteins with an average of 5780 proteins per sample. Compared to control kidneys, 614 proteins were up-regulated and 855 proteins were down-regulated (p value < 0.05, q value < 0.05, and fold change > 2 or < 0.5) in human obstructed kidneys. The up-regulated proteins were significantly enriched in collagen formation and immune response. Moreover, the human and mouse kidney proteomics reveled an aberrant NAD+ metabolism and NAD+-related metabolic pathways, including mitochondrial dysfunction, oxidative phosphorylation, and tryptophan metabolism. The expression patterns of several key enzymes catalyzing NAD+ biosynthesis and consumption were dramatically altered in obstructive nephropathy. Moreover, the major NAD+ consumer CD38 was strongly induced in obstructed kidneys which contributed to the decline of renal NAD+ levels. Functional studies demonstrated that CD38 deletion or inhibition significantly recovered renal NAD+ levels and reduced obstruction-induced renal fibrosis.
HostingRepository	iProX
AnnounceDate	2023-01-08
AnnouncementXML	Submission_2024-03-20_00:14:07.637.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jifeng Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-08 19:59:07	ID requested
⏵ 1	2024-03-20 00:14:08	announced

Tao Y, Wang J, Lyu X, Li N, Lai D, Liu Y, Zhang X, Li P, Cao S, Zhou X, Zhao Y, Ma L, Tao T, Feng Z, Li X, Yang F, Zhou H, Decline Orchestrating Renal Fibrosis in Pediatric Patients With Obstructive Nephropathy. Mol Cell Proteomics, 22(3):100510(2023) [pubmed]

submitter keyword: Obstructive nephropathy, Renal fibrosis, Kidney proteomics, CD38

Fuquan Yang
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	fqyang@ibp.ac.cn
lab head
Jifeng Wang
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	wangjifeng@ibp.ac.cn
dataset submitter

iProX dataset URI