PXD039309

PXD039309 is an original dataset announced via ProteomeXchange.

Title	Tracing retinal progenitors cells and photoreceptors from mouse retinal organoids
Description	Retinal organoids have become valuable 3D model for translational and developmental research. The knowledge of the limitations of the system ensures its sensible use. All retinal cell types originate from the differentiation of retinal progenitor cells. The properties of retinal progenitors in 3D culture systems are not well studied. In our project, we created a mouse stem cell line with a Rax-mCherry reporter construct that allows retinal progenitors' isolation, tracing, and in vitro imaging during retinogenesis. For proteomic analysis, we used mCherry-positive cells from dissociated embryoid bodies with formed eye field structures on the fourth day after stem cell aggregation. Information about protein content helped to characterize Rax-expressing cells at this stage and their suitability for further applications.
HostingRepository	PRIDE
AnnounceDate	2025-02-14
AnnouncementXML	Submission_2025-02-14_00:15:06.349.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfredo Quadroni
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-06 07:11:56	ID requested
⏵ 1	2025-02-14 00:15:06	announced

10.3390/CELLS14040252;

submitter keyword: retinal organoid, mouse embryonic stem cells, retinal progenitor cells

Yvan Arsenijevic
contact affiliation	Group “Retinal degeneration and regeneration” Department of Ophthalmology University of Lausanne Hôpital Ophtalmique Jules-Gonin Fundamental research center 15 av. de France 1004 Lausanne Switzerland
contact email	yvan.arsenijevic@fa2.ch
lab head
Manfredo Quadroni
contact affiliation	University of Lausanne
contact email	manfredo.quadroni@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD039309

PRIDE project URI

• PRIDE
  1. PXD039309
     1. Label: PRIDE project
     2. Name: Tracing retinal progenitors cells and photoreceptors from mouse retinal organoids