PXD039305

PXD039305 is an original dataset announced via ProteomeXchange.

Title	Eugenol modulates the NOD1- NF-κB signaling pathway via targeting NF-κB protein in triple breast cancer cells
Description	Background: The most aggressive subtype of breast cancer, triple-negative breast cancer (TNBC), has a worse prognosis and a higher probability of relapse since there is a narrow range of treatment options. Identifying and testing potential therapeutic targets for the treatment of TNBC is of high priority. Methods: Using a transcriptional signature of triple-negative breast cancer collected from Gene Expression Omnibus (GEO), CMap was utilized to reposition compounds for the treatment of TNBC. CCK8 and colony formation experiments were performed to detect the effect of the candidate drug on the proliferation of TNBC cells, while flow cytometry was used to detect cell apoptosis. Meanwhile, transwell assay was implemented to detect cell metastasis change caused by the candidate drug. Moreover, the proteomic approach was presently ongoing to evaluate the underlying mechanism of the candidate drug in TNBC. Furthermore, drug affinity responsive target stability (DARTS) coupled with LC-MS/MS was carried out to explore the potential drug target candidate in TNBC cells. Results: We found that the most widely used medication, eugenol, reduced the growth and metastasis of TNBC cells. According to the underlying mechanism revealed by proteomics, eugenol could inhibit TNBC cell proliferation via the NOD1-NF-κB signaling pathway. DARTS experiment further revealed that eugenol may bind to NF-κB in TNBC cells. Concludes: Our findings pointed out that eugenol was a potential candidate drug for the treatment of TNBC.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:10.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shi xiaoyu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-06 06:48:06	ID requested
1	2023-05-10 13:56:02	announced
⏵ 2	2023-11-14 08:47:11	announced	2023-11-14: Updated project metadata.

Shi X, Zhang W, Bao X, Liu X, Yang M, Yin C, B protein in triple-negative breast cancer cells. Front Endocrinol (Lausanne), 14():1136067(2023) [pubmed]

submitter keyword: proteomics, target protein.,eugenol, CMap, DARTs, triple-negative breast cancer

chengliang yin
contact affiliation	Macau University of Science and Technology, Faculty of Medicine, Macau, Macau SAR China
contact email	chengliangyin@163.com
lab head
Shi xiaoyu
contact affiliation	Yantai university
contact email	xiaoyushi1995@163.COM
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039305
     1. Label: PRIDE project
     2. Name: Eugenol modulates the NOD1- NF-κB signaling pathway via targeting NF-κB protein in triple breast cancer cells