PXD039275
PXD039275 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteins interacting with B2M in mouse synaptosome fractions |
Description | pGEX-4T-1-B2M was constructed using the GST gene fusion system according to the manufacturer’s instructions. To produce glutathione S-transferase (GST) and GST-B2M fusion proteins, pGEX-4T-1 and pGEX-4T-1-B2M were individually transfected into BL21 cells. Protein expression was induced by ispropylb-D-1-thiogalactopyranoside (IPTG). GST-B2M and GST proteins were purified by binding to Glutathione Sepharose-4B beads. Then, beads were incubated with mouse synaptosome fractions from C57BL/6J mice at 4 °C for 12 h on a rotator, and then were washed with the washing buffer three times. Beads-bound complexes were eluted by boiling, and subjected to SDS-PAGE and silver staining. |
HostingRepository | iProX |
AnnounceDate | 2023-01-04 |
AnnouncementXML | Submission_2023-02-23_19:13:26.627.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yue Gao |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-01-05 02:53:26 | ID requested | |
⏵ 1 | 2023-02-23 19:13:27 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: B2M, synaptosome, LC-MS |
Contact List
Xin Wang | |
---|---|
contact affiliation | Xiamen University |
contact email | wangx@xmu.edu.cn |
lab head | |
Yue Gao | |
contact affiliation | Xiamen University |
contact email | 1615766460@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |