PXD039274

PXD039274 is an original dataset announced via ProteomeXchange.

Title	Proteins interacting with B2M in mouse brain lysate
Description	pGEX-4T-1-B2M was constructed using the GST gene fusion system according to the manufacturer’s instructions. To produce glutathione S-transferase (GST) and GST-B2M fusion proteins, pGEX-4T-1 and pGEX-4T-1-B2M were individually transfected into BL21 cells. Protein expression was induced by ispropylb-D-1-thiogalactopyranoside (IPTG). GST-B2M and GST proteins were purified by binding to Glutathione Sepharose-4B beads. Then, beads were incubated with mouse brain lysates from C57BL/6J mice at 4 °C for 12 h on a rotator, and then were washed with the washing buffer three times. Beads-bound complexes were eluted by boiling, and subjected to SDS-PAGE and silver staining. The proteins were identified by Mass Spectrometry.
HostingRepository	iProX
AnnounceDate	2023-01-04
AnnouncementXML	Submission_2023-02-23_19:13:13.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Gao
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-05 02:48:53	ID requested
⏵ 1	2023-02-23 19:13:14	announced

Dataset with its publication pending

submitter keyword: B2M, brain lysate, LC-MS

Xin Wang
contact affiliation	Xiamen University
contact email	wangx@xmu.edu.cn
lab head
Yue Gao
contact affiliation	Xiamen University
contact email	1615766460@qq.com
dataset submitter

iProX dataset URI