PXD039257

PXD039257 is an original dataset announced via ProteomeXchange.

Title	Characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts
Description	In mammals, the neonatal heart regenerates within a short time after birth, but adults lack this ability. We have shown that metabolic reprogramming is critical for cardiomyocyte proliferation in the neonatal heart. Herein, we revealed that cardiac metabolic reprogramming could be regulated by altering global protein lactylation. 4D label-free proteomics and Kla omics were performed in postnatal Day 1 (P1), 5 (P5), and 7 (P7) mouse hearts, 2297 Kla sites from 980 proteins were identified, and 1262 Kla sites from 409 proteins were quantified. Functional clustering analysis of proteins with altered Kla sites revealed that the proteins were mainly involved in metabolic processes. The Kla levels in several fatty acid oxidation-related proteins showed high expression at P5, while glycolysis and cell cycle-related proteins were sustainedly decreased from P1-P7. Furthermore, we verified the Kla levels of several differentially modified proteins, including ACAT1, ACADL, PKM and NPM1, by coimmunoprecipitation and Western blotting. Overall, we reported the first comprehensive Kla map in the neonatal mouse heart, which will aid in understanding the regulatory network of metabolic reprogramming and cardiac regeneration.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:48:01.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tongyu Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	lactic acid
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-04 04:31:35	ID requested
1	2024-07-01 20:26:14	announced
⏵ 2	2024-10-22 06:48:02	announced	2024-10-22: Updated project metadata.

10.1016/j.jgg.2024.02.009;

Zhang T, Zhu Y, Wang X, Chong D, Wang H, Bu D, Zhao M, Fang L, Li C, The characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts. J Genet Genomics, 51(7):735-748(2024) [pubmed]

submitter keyword: Cell proliferation, Metabolic reprogramming, Postnatal heart regeneration,Lactylation

Chaojun Li
contact affiliation	State Key Laboratory of Reproductive Medicine and China International Joint Research Center on Environment and Human Health, Nanjing Medical University
contact email	lichaojun@njmu.edu.cn
lab head
Tongyu Zhang
contact affiliation	Nanjing University
contact email	zhangty@nicemice.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD039257

PRIDE project URI

• PRIDE
  1. PXD039257
     1. Label: PRIDE project
     2. Name: Characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts