PXD039240

PXD039240 is an original dataset announced via ProteomeXchange.

Title	Evaluation of depletion of abundant plasma proteins for proteomics characterization of EVs
Description	In this study, we aimed to investigate if the removal of abundant plasma proteins prior to EV isolation could improve plasma-derived EV characterization by LC-MS/MS by improving the proteome coverage. Plasma depletion was performed using single-use spin columns prior to SEC-based EV isolation. Afterwards, EVs derived from undepleted and depleted plasma were characterized by mass spectrometry (MS)-based proteomics using a data-independent acquisition (DIA) approach.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:09.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandrine Reymond
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-04 03:50:49	ID requested
1	2023-07-20 11:14:55	announced
⏵ 2	2023-11-14 09:03:15	announced	2023-11-14: Updated project metadata.

Reymond S, Gruaz L, Sanchez JC, Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls. Anal Bioanal Chem, 415(16):3177-3187(2023) [pubmed]

submitter keyword: EV, Plasma depletion, DIA,Extracellular vesicles, LC-MS/MS

Jean-Charles Sanchez
contact affiliation	Translational Biomarker Group, Department of Medicine, University of Geneva
contact email	jean-charles.sanchez@unige.ch
lab head
Sandrine Reymond
contact affiliation	University of Geneva
contact email	sandrine.reymond@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD039240

PRIDE project URI

• PRIDE
  1. PXD039240
     1. Label: PRIDE project
     2. Name: Evaluation of depletion of abundant plasma proteins for proteomics characterization of EVs