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PXD039237

PXD039237 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted exercise enhances functional integration of engineered muscle grafts after volumetric muscle loss
DescriptionDamage that affects large volumes of skeletal muscle tissue can severely impact health, mobility, and quality-of-life. Efforts to restore muscle function by implanting engineered grafts at the site of damage have demonstrated limited restoration of force production. Various forms of mechanical and biochemical stimulation have been shown to have a potentially beneficial impact on muscle maturation, vascularization, and innervation, but yield unpredictable and inconsistent recovery of functional mobility. Here we show that targeted exercise of optogenetic engineered muscle grafts restores motor functions 2 weeks post-injury. Furthermore, we conduct phosphoproteomic analysis of grafts in vitro and in vivo to show that exercise training alters signaling pathways that play key roles in skeletal muscle contractility, neurite growth, neuromuscular synapse formation, angiogenesis, and cytoskeletal remodeling. Our study uncovers several proteins not previously known to be modulated by exercise, revealing promising mechanisms for leveraging targeted exercise to enhance functional integration of tissue engineered muscle.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_09:11:53.868.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD039237
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTigist Tamir
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentOrbitrap Exploris 480; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-04 03:30:54ID requested
12025-05-06 09:11:54announced
Publication List
10.6019/PXD039237;
10.1016/j.biomaterials.2023.122317;
Rousseau E, Raman R, Tamir T, Bu A, Srinivasan S, Lynch N, Langer R, White FM, Cima MJ, Actuated tissue engineered muscle grafts restore functional mobility after volumetric muscle loss. Biomaterials, 302():122317(2023) [pubmed]
Keyword List
submitter keyword: engineered muscle graft, muscle phosphoproteomics, volumetric muscle loss
Contact List
Forest M White
contact affiliationMassachusetts Institute of Technology, Department of Biological Engineering, David H Koch Institute for Integrative Cancer Research
contact emailfwhite@mit.edu
lab head
Tigist Tamir
contact affiliationMassachusetts Institute of Technology, David H Koch Institute for Integrative Cancer Research
contact emailtytamir@mit.edu
dataset submitter
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Dataset FTP location
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