PXD039237

PXD039237 is an original dataset announced via ProteomeXchange.

Title	Targeted exercise enhances functional integration of engineered muscle grafts after volumetric muscle loss
Description	Damage that affects large volumes of skeletal muscle tissue can severely impact health, mobility, and quality-of-life. Efforts to restore muscle function by implanting engineered grafts at the site of damage have demonstrated limited restoration of force production. Various forms of mechanical and biochemical stimulation have been shown to have a potentially beneficial impact on muscle maturation, vascularization, and innervation, but yield unpredictable and inconsistent recovery of functional mobility. Here we show that targeted exercise of optogenetic engineered muscle grafts restores motor functions 2 weeks post-injury. Furthermore, we conduct phosphoproteomic analysis of grafts in vitro and in vivo to show that exercise training alters signaling pathways that play key roles in skeletal muscle contractility, neurite growth, neuromuscular synapse formation, angiogenesis, and cytoskeletal remodeling. Our study uncovers several proteins not previously known to be modulated by exercise, revealing promising mechanisms for leveraging targeted exercise to enhance functional integration of tissue engineered muscle.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:11:53.868.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039237
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tigist Tamir
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-04 03:30:54	ID requested
⏵ 1	2025-05-06 09:11:54	announced

10.6019/PXD039237;

10.1016/j.biomaterials.2023.122317;

Rousseau E, Raman R, Tamir T, Bu A, Srinivasan S, Lynch N, Langer R, White FM, Cima MJ, Actuated tissue engineered muscle grafts restore functional mobility after volumetric muscle loss. Biomaterials, 302():122317(2023) [pubmed]

submitter keyword: engineered muscle graft, muscle phosphoproteomics, volumetric muscle loss

Forest M White
contact affiliation	Massachusetts Institute of Technology, Department of Biological Engineering, David H Koch Institute for Integrative Cancer Research
contact email	fwhite@mit.edu
lab head
Tigist Tamir
contact affiliation	Massachusetts Institute of Technology, David H Koch Institute for Integrative Cancer Research
contact email	tytamir@mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD039237

PRIDE project URI

• PRIDE
  1. PXD039237
     1. Label: PRIDE project
     2. Name: Targeted exercise enhances functional integration of engineered muscle grafts after volumetric muscle loss