PXD039237 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted exercise enhances functional integration of engineered muscle grafts after volumetric muscle loss |
Description | Damage that affects large volumes of skeletal muscle tissue can severely impact health, mobility, and quality-of-life. Efforts to restore muscle function by implanting engineered grafts at the site of damage have demonstrated limited restoration of force production. Various forms of mechanical and biochemical stimulation have been shown to have a potentially beneficial impact on muscle maturation, vascularization, and innervation, but yield unpredictable and inconsistent recovery of functional mobility. Here we show that targeted exercise of optogenetic engineered muscle grafts restores motor functions 2 weeks post-injury. Furthermore, we conduct phosphoproteomic analysis of grafts in vitro and in vivo to show that exercise training alters signaling pathways that play key roles in skeletal muscle contractility, neurite growth, neuromuscular synapse formation, angiogenesis, and cytoskeletal remodeling. Our study uncovers several proteins not previously known to be modulated by exercise, revealing promising mechanisms for leveraging targeted exercise to enhance functional integration of tissue engineered muscle. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_09:11:53.868.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD039237 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Tigist Tamir |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Exploris 480; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-04 03:30:54 | ID requested | |
⏵ 1 | 2025-05-06 09:11:54 | announced | |
Publication List
10.6019/PXD039237; |
10.1016/j.biomaterials.2023.122317; |
Rousseau E, Raman R, Tamir T, Bu A, Srinivasan S, Lynch N, Langer R, White FM, Cima MJ, Actuated tissue engineered muscle grafts restore functional mobility after volumetric muscle loss. Biomaterials, 302():122317(2023) [pubmed] |
Keyword List
submitter keyword: engineered muscle graft, muscle phosphoproteomics, volumetric muscle loss |
Contact List
Forest M White |
contact affiliation | Massachusetts Institute of Technology, Department of Biological Engineering, David H Koch Institute for Integrative Cancer Research |
contact email | fwhite@mit.edu |
lab head | |
Tigist Tamir |
contact affiliation | Massachusetts Institute of Technology, David H Koch Institute for Integrative Cancer Research |
contact email | tytamir@mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD039237
- Label: PRIDE project
- Name: Targeted exercise enhances functional integration of engineered muscle grafts after volumetric muscle loss