PXD039221

PXD039221 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Tissue-regulated collagen hydroxylation at GEP/GDP motifs mediated by P4HA2
Description	Collagen, the most abundant organic compound of vertebrate organisms, is a supramolecular protein-made polymer. Details of its subtle post-translational maturation largely determine the mechanical properties of connective tissues. Its assembly requires massive, heterogeneous prolyl-4-hydroxylation (P4H), catalyzed by Prolyl-4-hydroxylases (P4HA1-3), providing thermostability to its elemental, triple helical building block. So far, there was no evidence of tissue-specific regulation of P4H, nor of a differential substrate repertoire of P4HAs. Here, the positional P4H profiles of collagen extracted from bone, skin, and tendon were compared, revealing lower hydroxylation of most GEP/GDP motifs in the tendon, across homeotherms. P4HA2 mRNA was found low in tendon. Invalidation of P4HA2, unlike decreased, generalized P4H activity, in the ATDC5 cellular model of collagen assembly, mimicked the tendon-related P4H profile. Therefore, P4HA2 has a better ability than other P4HAs to hydroxylate GEP/GDP motifs and the differential expression of P4HAs in tissues dictates the positional P4H profile of collagen, which participates in determining tissue specificities of its assembly.
HostingRepository	MassIVE
AnnounceDate	2023-03-29
AnnouncementXML	Submission_2023-03-29_04:02:30.580.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	vincourt
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; scientific name: Gallus gallus; common name: chicken; NCBI TaxID: 9031;
ModificationList	Oxidation
Instrument	autoflex TOF/TOF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-03 05:27:25	ID requested
⏵ 1	2023-03-29 04:02:30	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: type I collagen, 4-hydroxyproline, prolyl-4-hydroxylase, tendon

submitter keyword: type I collagen, 4-hydroxyproline, prolyl-4-hydroxylase, tendon

Contact List

vincourt
contact affiliation	IMoPA UMR 7365 CNRS UL
contact email	jean-baptiste.vincourt@univ-lorraine.fr
lab head
vincourt
contact affiliation	proteomics IBSLor
contact email	jean-baptiste.vincourt@univ-lorraine.fr
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091002/