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PXD039212

PXD039212 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic Characterization of human small airway epithelial cells (hSAECs) in the context of respiratory syncytial virus infection and BRD4 inhibition.
DescriptionBromodomain-containing Protein 4 (BRD4) is a transcriptional regulator associated with cancer biology, inflammation, and fibrosis. In airway viral infection, non-toxic BRD4-specific inhibitors (BRD4i) block the release of pro- inflammatory cytokines and prevent downstream remodeling. Although the chromatin modifying functions of BRD4 in inducible gene expression have been extensively investigated, its roles in post-transcriptional regulation are not as well understood. Based on its interaction with transcriptional elongation complex and spiceosome, we hypothesize that BRD4 is a functional regulator of RNA processing. To address this question, we combine data-independent analysis - parallel accumulation-serial fragmentation (diaPASEF) with RNA-sequencing to achieve deep coverage of the proteomic and transcriptomic landscape of human small airway epithelial cells exposed to viral challenge and treated with BRD4i. The transcript-level data was further interrogated for alternative splicing analysis, and the resulting data sets were correlated to identify pathways subject to post-transcriptional regulation. We discover that BRD4 regulates alternative splicing of key genes, including Interferon-related Developmental Regulator 1 (IFRD1) and X-Box Binding Protein 1 (XBP1), related to the innate immune response and the unfolded protein response, respectively. These findings extend the actions of BRD4 in control of post-transcriptional RNA processing.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:58:35.573.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMorgan Mann
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentBruker Daltonics timsTOF series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-03 01:00:06ID requested
12023-06-12 21:21:01announced
22023-11-14 08:58:36announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human, Epithelial, LC-MS, Airway, diaPASEF
Contact List
Allan R. Brasier
contact affiliationInstitute for Clinical and Translational Research, University of Wisconsin - Madison
contact emailabrasier@wisc.edu
lab head
Morgan Mann
contact affiliationUW-Madison
contact emailmwmann@wisc.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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