PXD039211

PXD039211 is an original dataset announced via ProteomeXchange.

Title	Metalloprotease-mediated cleavage of CD95 Ligand: QTOF Xevo XS data
Description	CD95 is a member of the TNF receptor superfamily that is ubiquitously expressed in healthy and pathological tissues. Stimulation of CD95 by its physiological ligand CD95L induces its oligomerization leading in turn to the transduction of either apoptotic or non-apoptotic signals. CD95L can exist as both membrane-anchored and soluble forms (sCD95L), the latter resulting from the proteolytic cleavage of the former. Candidate proteases able to achieve CD95L cleavage were identified as matrix metalloproteases (MMP) due to their demonstrated ability to cleave other TNF superfamily ligands. The main goal of this study was to systematically identify the MMP family members capable of cleaving CD95L and subsequently determine the corresponding cleavage sites. By using different orthogonal biochemical approaches and combining them with molecular modeling, we confirmed data from the literature regarding CD95L cleavage by MMP-3 and MMP-7. Moreover, we found that MMP-2 and MMP-12 can cleave CD95L and characterized their resulting cleavage sites. This study provides a systematic approach to analyze the cleavage of CD95L, which until now had only been poorly described.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:30:36.487.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Negroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Xevo G2-XS QTof

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-03 00:59:50	ID requested
1	2023-02-20 06:32:43	announced
⏵ 2	2023-11-14 08:30:37	announced	2023-11-14: Updated project metadata.

Risso V, Thomas M, Gu, é, vel B, Lavigne R, Com E, Martin S, Nivet M, Pineau C, Negroni L, Lafont É, Chevet É, Eriksson LA, Le Gallo M, Metalloprotease-mediated cleavage of CD95 ligand. FEBS J, 290(12):3145-3164(2023) [pubmed]

submitter keyword: CD95L,Metalloprotease, proteolytic cleavage

Eric Chevet
contact affiliation	Inserm UMR_S 1242, Chemistry Oncogenesis Stress Signaling, University of Rennes, Rennes,France.
contact email	eric.chevet@inserm.fr
lab head
Luc Negroni
contact affiliation	CNRS
contact email	luc.negroni@igbmc.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039211

PRIDE project URI

• PRIDE
  1. PXD039211
     1. Label: PRIDE project
     2. Name: Metalloprotease-mediated cleavage of CD95 Ligand: QTOF Xevo XS data