PXD039208

PXD039208 is an original dataset announced via ProteomeXchange.

Title	Label-Free Single Cell Proteomics Utilizing Ultrafast LC and MS Instrumentation: A Valuable Complementary Technique to Multiplexing.
Description	In recent years, single cell proteomics became one of the hottest topics within the field of proteomics. The potential ability to map the proteomic fingerprint to transcriptomic data would master the understanding of how gene expression translates into actual phenotypes and cellular functions. In contrast to nucleic acid sequencing, in vitro amplification of proteins is impossible to date and no single cell proteomic workflow has been established as gold standard yet. Lots of advances in microfluidic sample preparation, multi-dimensional sample separation, sophisticated data acquisition strategies and intelligent data analysis algorithms already brought major improvements to successfully analyze such tiny sample amounts with steadily boosted performance. However, among the broad variation of published approaches, its commonly accepted, that highest possible sensitivity, robustness and throughput are still the most urgent needs for the field. While many labs have focused on multiplexing to achieve these goals, label-free single cell proteomics is a highly promising strategy as well whenever high dynamic range and unbiased accurate quantification are needed. We here focus on recent advances of label-free single-cell mass spectrometry workflows and try to guide our readers to choose the best method or method combinations for their specific application. We further highlight which techniques are most propitious in the future and which applications but also limitations we foresee for the field.
HostingRepository	PRIDE
AnnounceDate	2023-02-09
AnnouncementXML	Submission_2023-02-09_09:36:49.022.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ManuelMatzinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-03 00:56:25	ID requested
⏵ 1	2023-02-09 09:36:49	announced

Dataset with its publication pending

submitter keyword: DIA,label-free-proteomics, data-completness, DDA

KarlMechtler
contact affiliation	Research Institute for Molecular Pathology
contact email	karl.mechtler@imp.ac.at
lab head
ManuelMatzinger
contact affiliation	IMP / University of Vienna
contact email	manuel.matzinger@imp.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039208

PRIDE project URI

• PRIDE
  1. PXD039208
     1. Label: PRIDE project
     2. Name: Label-Free Single Cell Proteomics Utilizing Ultrafast LC and MS Instrumentation: A Valuable Complementary Technique to Multiplexing.