PXD039206 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics analysis of the effects of Grammatophyllum speciosum extracts on macrophage cells |
Description | Grammatophyllum speciosum is a traditional plant with beneficial functionalities for health. G. speciosum extracts can inhibit collagenase and nitric oxide without cellular toxicity in keratinocytes. The extracts have shown potential for use and formulation as cosmeceutical ingredients. However, the molecular mechanisms underlying these activities remain unknown. In this dataset, we used a proteomics approach to clarify the proteins that participate in the response of RAW264.7 macrophage cells to G. speciosum extracts. Cells were divided into two experimental groups, i.e., the control and treatment groups. In turn, the treatment group included two subgroups that were treated with 20 and 100 g/mL of the extracts, respectively. The experiments were conducted using two biological replicates. The dataset was obtained from label-free proteomics using high-resolution LC-MS/MS with four technical replicates. The quality control (QC) of the proteomics dataset was carried out using chromatography at the MS1 and MS2 levels, peptide mass deviation, peptide mass cleavage, sequence length, and total peptide intensity. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-03 |
AnnouncementXML | Submission_2023-01-03_05:01:53.437.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD039206 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | SucheewinKrobthong |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-03 00:55:47 | ID requested | |
⏵ 1 | 2023-01-03 05:01:53 | announced | |
Publication List
Keyword List
submitter keyword: LC-MS/MS, RAW264.7, traditional plant, cosmeceuticals |
Contact List
SucheewinKrobthong |
contact affiliation | Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand |
contact email | sucheewin82@gmail.com |
lab head | |
SucheewinKrobthong |
contact affiliation | Mahidol University |
contact email | sucheewin82@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039206
- Label: PRIDE project
- Name: Proteomics analysis of the effects of Grammatophyllum speciosum extracts on macrophage cells