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PXD039206

PXD039206 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics analysis of the effects of Grammatophyllum speciosum extracts on macrophage cells
DescriptionGrammatophyllum speciosum is a traditional plant with beneficial functionalities for health. G. speciosum extracts can inhibit collagenase and nitric oxide without cellular toxicity in keratinocytes. The extracts have shown potential for use and formulation as cosmeceutical ingredients. However, the molecular mechanisms underlying these activities remain unknown. In this dataset, we used a proteomics approach to clarify the proteins that participate in the response of RAW264.7 macrophage cells to G. speciosum extracts. Cells were divided into two experimental groups, i.e., the control and treatment groups. In turn, the treatment group included two subgroups that were treated with 20 and 100 g/mL of the extracts, respectively. The experiments were conducted using two biological replicates. The dataset was obtained from label-free proteomics using high-resolution LC-MS/MS with four technical replicates. The quality control (QC) of the proteomics dataset was carried out using chromatography at the MS1 and MS2 levels, peptide mass deviation, peptide mass cleavage, sequence length, and total peptide intensity.
HostingRepositoryPRIDE
AnnounceDate2023-01-03
AnnouncementXMLSubmission_2023-01-03_05:01:53.437.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD039206
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSucheewinKrobthong
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-03 00:55:47ID requested
12023-01-03 05:01:53announced
Publication List
10.6019/PXD039206;
Keyword List
submitter keyword: LC-MS/MS, RAW264.7, traditional plant, cosmeceuticals
Contact List
SucheewinKrobthong
contact affiliationDepartment of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
contact emailsucheewin82@gmail.com
lab head
SucheewinKrobthong
contact affiliationMahidol University
contact emailsucheewin82@gmail.com
dataset submitter
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Dataset FTP location
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