PXD039191

PXD039191 is an original dataset announced via ProteomeXchange.

Title	Yeast Mitochondrial Derived Vesicles (MDVs) have membrane potential and contain a functional ATP synthase-Part 2
Description	Mitochondria are responsible for the production of energy and for essential metabolic pathways in the cell, as well as fundamental processes such as apoptosis and aging. Interestingly, while the import machinery of proteins into mitochondria is thoroughly understood, there is currently no known protein export mechanism from mitochondria. Vesicular transport is a means of inter-organellar communication, and in fact cells release vesicles in order to shuttle lipids, proteins, RNA and DNA between one another. Here we show for the first time that functional mitochondria, isolated from Saccharomyces cerevisiae can release vesicles, independent of the fission machinery. Mitochondrial secreted vesicles are relatively uniform in size, of about 100nm and carry a selection of cargo; only certain proteins are carried by these vesicles, of which some are matrix soluble and some are membrane proteins. We further demonstrate that yeast MDVs harbor a membrane potential, a functional ATP synthase complex, produce ATP, and fuse with naïve mitochondria. We suggest, that MDVs may have a function in organellar protein export, ATP production ability of damaged mitochondria and organelle-communication.
HostingRepository	PRIDE
AnnounceDate	2023-01-29
AnnouncementXML	Submission_2023-01-28_23:58:21.172.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TamarZiv
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 19:16:13	ID requested
⏵ 1	2023-01-28 23:58:21	announced

Dataset with its publication pending

submitter keyword: Vesicles, ATP synthase, Yeast Mitochondria

PinesOphry
contact affiliation	Department of Molecular Genetics and Microbiology, IMRIC, Faculty of Medicine, Hebrew University, Jerusalem, Israel
contact email	ophryp@ekmd.huji.ac.il
lab head
TamarZiv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD039191

PRIDE project URI

• PRIDE
  1. PXD039191
     1. Label: PRIDE project
     2. Name: Yeast Mitochondrial Derived Vesicles (MDVs) have membrane potential and contain a functional ATP synthase-Part 2