PXD039179

PXD039179 is an original dataset announced via ProteomeXchange.

Title	Chromatome of AR and SUMO2/3 idenitified with RIME
Description	We identifyied AR and SUMO2/3 chromatomes in castration-resistant prostate cancer cells (VCaP) and studied the impact on SUMOylation inhibition (SUMOi)on them utilizing small molecule inhibitor ML792. Chromatome members were identifyied with Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) in absence and presence of androgen and ML792. Mass spectrometry analyses were executed with high resolution mass spectrometry LC-MS analysis was performed by using the Evosep One liquid chromatography system coupled to a hybrid trapped ion mobility quadrupole TOF mass spectrometer (Bruker timsTOF Pro) via a CaptiveSpray nano-electrospray ion source. High-confidence chromatome members discriminated from background with SAINT analysis.
HostingRepository	PRIDE
AnnounceDate	2024-08-08
AnnouncementXML	Submission_2024-08-08_02:39:13.543.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaisa-Mari Launonen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 19:10:57	ID requested
⏵ 1	2024-08-08 02:39:13	announced

10.1093/NAR/GKAE653;

submitter keyword: AR, SUMOylation, RIME, chromatome, Bruker timsTOF Pro

Jorma Palvimo
contact affiliation	Professor of Medical Biochemistry, Institute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland
contact email	jorma.palvimo@uef.fi
lab head
Kaisa-Mari Launonen
contact affiliation	PhD student
contact email	kaisa-mari.launonen@uef.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039179
     1. Label: PRIDE project
     2. Name: Chromatome of AR and SUMO2/3 idenitified with RIME