PXD039174

PXD039174 is an original dataset announced via ProteomeXchange.

Title	High Field Asymmetric Waveform Ion Mobility Spectrometry- Mass Spectrometry to Enhance Cardiac Muscle Proteome Coverage
Description	Heart tissue sample preparation for mass spectrometry (MS) analysis that includes pre-fractionation reduces the cellular protein dynamic range and increases the relative abundance of non-sarcomeric proteins. We previously described “IN-Sequence” (IN-Seq) where heart tissue lysate is sequentially partitioned into three subcellular fractions to increase the proteome coverage than a single direct tissue analysis by mass spectrometry. Here, we report an adaptation of the high-field asymmetric ion mobility spectrometry (FAIMS) coupled to mass spectrometry, and the establishment of a simple one step sample preparation coupled with gas-phase fractionation. FAIMS approach substantially reduces manual sample handling, significantly shortens MS instrument processing time, and produces unique protein identification and quantification approximating the commonly used IN-Seq method in for less time requirement.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:03.353.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandr Stotland
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 19:09:13	ID requested
1	2023-07-20 11:13:49	announced
⏵ 2	2023-11-14 09:03:05	announced	2023-11-14: Updated project metadata.

Ai L, Binek A, Kreimer S, Ayres M, Stotland A, Van Eyk JE, High-Field Asymmetric Waveform Ion Mobility Spectrometry: Practical Alternative for Cardiac Proteome Sample Processing. J Proteome Res, 22(6):2124-2130(2023) [pubmed]

submitter keyword: FAIMS, DIA-MS,Cardiac, IN-Seq

Jennifer Van Eyk
contact affiliation	Cedars-Sinai Medical Center, Heart Institute Advanced Clinical Biosystems Research Institute
contact email	jennifer.vaneyk@cshs.org
lab head
Aleksandr Stotland
contact affiliation	Cedars-Sinai Medical Center
contact email	aleksandr.stotland@cshs.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD039174

PRIDE project URI

• PRIDE
  1. PXD039174
     1. Label: PRIDE project
     2. Name: High Field Asymmetric Waveform Ion Mobility Spectrometry- Mass Spectrometry to Enhance Cardiac Muscle Proteome Coverage