PXD039170

PXD039170 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of High-grade glioma tissues showing differential fluorescence following 5-Aminolevulinic acid guided surgery
Description	Targeted treatment of high-grade gliomas (HGGs) is challenging due to intra- and inter-tumoral heterogeneity. Prognosis of these tumors relies largely on the extent of resection. Fluorescence guided surgery using 5-ALA as adjunct has been on the rise in the recent years. However, 5-ALA has been ineffective in a small subset of population with similar histological phenotypes but varying metabolic/biochemical properties. Visualized fluorescence can sometimes be subjective and lead to variability in defining fluorescing regions with respect to their biological grade. Objective assessment of fluorescence is possible using spectroscopic techniques and with ex vivo PpIX assessment assays. The biometric study in our previous work revealed that even with objective assessment using PpIX assays, there exists a small subpopulation of tumor cells with similar histological phenotypes but discordant metabolic/biochemical properties w.r.t accumulation of PpIX. In the current study, we extended the investigation further and have carried out proteomic analysis of high-grade glioma tissue samples resected using 5-ALA fluorescence guided surgery to understand molecular differences leading to differential fluorescence in these complex and heterogenous tumors.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:56:44.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Saicharan Ghantasala
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 19:08:00	ID requested
1	2023-07-20 11:53:46	announced
2	2023-11-14 09:03:45	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:56:50	announced	2024-10-22: Updated project metadata.

10.1093/noajnl/vdad065;

Ghantasala S, Bhat A, Agarwal U, Biswas D, Bhattarai P, Epari S, Moiyadi A, Srivastava S, Deep proteome investigation of high-grade gliomas reveals heterogeneity driving differential metabolism of 5-aminolevulinic acid. Neurooncol Adv, 5(1):vdad065(2023) [pubmed]

submitter keyword: Deep proteome, Protoporphyrin IX,5-Aminolevulinic acid, Gene Set Enrichment Analysis, Heme Biosynthesis, LC-MS, High-grade gliomas

Prof. Sanjeeva Srivastava
contact affiliation	Biosciences and Bioengineering, IIT Bombay, India
contact email	sanjeeva@iitb.ac.in
lab head
Saicharan Ghantasala
contact affiliation	Indian Institute of Technology Bombay
contact email	saicharan.ghantasala@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD039170

PRIDE project URI

• PRIDE
  1. PXD039170
     1. Label: PRIDE project
     2. Name: Proteomics analysis of High-grade glioma tissues showing differential fluorescence following 5-Aminolevulinic acid guided surgery