PXD039161

PXD039161 is an original dataset announced via ProteomeXchange.

Title	human endometrial proteomics analysis
Description	Competent endometrial receptivity is a prerequisite for successful embryo implantation. Identification of novel key molecules involved in endometrial receptivity is essential to better interpret human implantation and improve pregnancy rates in assisted reproduction treatment. The isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics was performed to profile the proteomes of the prereceptive( LH+2, n=4) and receptive (LH+7, n=4) endometrial tissues. One hundred seventy-three differentially expressed proteins (DEPs) between LH+2 and LH+7 endometrial samples were identified. Integrated analysis of the proteomic data and published transcriptomic data was performed to identify the concordant DEPs with differential expression at both mRNA and protein levels. The protein-protein-interaction (PPI) network analysis were performed on concordant DEPs.We first identified 63 novel concordant DEPs and 5 hub proteins (ACSL4, ACSL5, COL1A1, PTGS1 and PLA2G4F) between LH+2 and LH+7 endometrial samples. ACSL4 was predominantly expressed in endometrial epithelial cells and its expression was significantly upregulated in the LH+7 endometrium and significantly downregulated in repeated implantation failure (RIF) patients. Knockdown of ACSL4 in endometrial epithelial cells induced the down-regulation of endometrial receptivity markers (HOXA10, COX2 and LIF) and the significant decrease of implantation rate during in vitro implantation analysis. This study provides the first gel-independent quantitative proteomes of the LH+2 and LH+7 human endometrium using iTRAQ technology. The identified concordant DEPs and hub proteins open a new avenue for future studies aimed at elucidating the underlying mechanisms governing endometrial receptivity. ACSL4 was identified as a novel regulatory molecule in the establishment of endometrial receptivity and might play important roles during implantation.
HostingRepository	iProX
AnnounceDate	2023-01-02
AnnouncementXML	Submission_2023-01-02_18:52:24.395.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuanggang Hu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 18:51:58	ID requested
⏵ 1	2023-01-02 18:52:24	announced

Dataset with its publication pending

submitter keyword: endometrial receptivity, implantation, proteome, iTRAQ, ACSL4

Yun Sun
contact affiliation	Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
contact email	syun163@163.com
lab head
Shuanggang Hu
contact affiliation	Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
contact email	shuangganghu@163.com
dataset submitter

iProX dataset URI