PXD039145

PXD039145 is an original dataset announced via ProteomeXchange.

Title	Can Blood Proteome Diversity Among Fish Species Help Explain Perfluoroalkyl Acid Trophodynamics in Aquatic Food Webs?
Description	Per- and polyfluoroalkyl substances (PFAS) are a diverse family of industrially significant synthetic chemicals infamous for extreme environmental persistence and global environmental distribution. Many PFAS are bioaccumulative and biologically active mainly due to their tendency to bind with various proteins. These protein interactions may be the most important element in determining the accumulation potential and tissue distribution of individual PFAS. Trophodynamics studies including aquatic food webs present inconsistent evidence for PFAS biomagnification. This study strives to identify whether the observed variability in PFAS bioaccumulation potential among species could correspond with interspecies protein composition differences. Specifically, this work compares the perfluorooctane sulfonate (PFOS) serum protein binding potential and the tissue distribution of ten perfluoroalkyl acids (PFAAs) detected in alewife (Alosa pseudoharengus), deepwater sculpin (Myoxocephalus thompsonii), and lake trout (Salvelinus namaycush) of the Lake Ontario aquatic piscivorous food web. To identify interspecies differences in PFAS-binding serum proteins, fish sera were pre-equilibrated with PFOS, fractionated by serial molecular weight cut-off filter fractionation, followed by liquid chromatography–tandem mass spectrometry analysis of the tryptic protein digests and the PFOS extracts of each fraction. This workflow identified similar serum proteins for all fish species. However, serum albumin was only identified in lake trout, suggesting apolipoproteins are likely the primary PFAA transporters in alewife and deepwater sculpin sera. PFAA tissue distribution analysis provided supporting evidence for interspecies variations in lipid transport and storage, which may also contribute to the varied PFAA accumulation in these species.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:53.951.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adam Point
SpeciesList	scientific name: Alosa pseudoharengus; NCBI TaxID: 34774; scientific name: Salvelinus namaycush; NCBI TaxID: 8040; scientific name: Myoxocephalus thompsonii; NCBI TaxID: 304292;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Xevo G2 Q-Tof

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 01:29:52	ID requested
1	2023-03-11 11:09:42	announced
⏵ 2	2023-11-14 08:43:54	announced	2023-11-14: Updated project metadata.

Point AD, Crimmins BS, Holsen TM, Fernando S, Hopke PK, Darie CC, Can blood proteome diversity among fish species help explain perfluoroalkyl acid trophodynamics in aquatic food webs? Sci Total Environ, 875():162337(2023) [pubmed]

submitter keyword: shotgun proteomics, apolipoproteins, biomagnification, protein binding,PFAS, albumin

Costel C. Darie
contact affiliation	Biochemistry & Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA
contact email	cdarie@clarkson.edu
lab head
Adam Point
contact affiliation	Clarkson University
contact email	apoint1292@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD039145

PRIDE project URI

• PRIDE
  1. PXD039145
     1. Label: PRIDE project
     2. Name: Can Blood Proteome Diversity Among Fish Species Help Explain Perfluoroalkyl Acid Trophodynamics in Aquatic Food Webs?