PXD039128 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomes of acrosomes and acrosome-reacted sperm of the house mouse |
Description | Using label-free nano-scale liquid chromatography tandem mass spectrometry (nLC-MS2) proteomics, we provide proteomes of the acrosomes and acrosome-reacted sperm isolated from cauda epididymis of free-living house mice Mus musculus musculus. Among all proteins, 334 proteins were significantly enriched in the acrosome thus representing 27.3% of the whole proteome of the intact sperm. Importantly, we have detected a total of nine calycins while eight of them belong to the lipocalin protein family. Column names in resulting tables are tagged as S1-S10 (supernatants) and represent acrosomal content (A1-A10 in Table S1) while samples P1-P10 are pellets and represent arcosome reacted sperm (S1-S10 in Table S1). |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:55:37.777.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pavel Stopka |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-02 01:16:33 | ID requested | |
1 | 2023-02-27 07:19:27 | announced | |
2 | 2023-02-28 06:35:14 | announced | 2023-02-28: Updated project metadata. |
⏵ 3 | 2023-11-14 07:55:38 | announced | 2023-11-14: Updated project metadata. |
Publication List
Ot, č, en, á, š, kov, á T, Mac, í, č, kov, á E, Vondr, á, kov, á J, Frol, í, kov, á M, Komrskova K, Stopkov, á R, Stopka P, Proteomic analysis of the mouse sperm acrosome - towards an understanding of an organelle with diverse functionality. Eur J Cell Biol, 102(2):151296(2023) [pubmed] |
Keyword List
submitter keyword: Mus musculus,acrosome, label free |
Contact List
Pavel Stopka |
contact affiliation | BIOCEV group, Department of Zoology, Faculty of Science, Charles University in Prague, Průmyslová 595, 252 42 Vestec |
contact email | pavel.stopka@natur.cuni.cz |
lab head | |
Pavel Stopka |
contact affiliation | Department of Zoology, Charles University, Prague |
contact email | pstopka@natur.cuni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039128 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039128
- Label: PRIDE project
- Name: Proteomes of acrosomes and acrosome-reacted sperm of the house mouse