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PXD039128

PXD039128 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomes of acrosomes and acrosome-reacted sperm of the house mouse
DescriptionUsing label-free nano-scale liquid chromatography tandem mass spectrometry (nLC-MS2) proteomics, we provide proteomes of the acrosomes and acrosome-reacted sperm isolated from cauda epididymis of free-living house mice Mus musculus musculus. Among all proteins, 334 proteins were significantly enriched in the acrosome thus representing 27.3% of the whole proteome of the intact sperm. Importantly, we have detected a total of nine calycins while eight of them belong to the lipocalin protein family. Column names in resulting tables are tagged as S1-S10 (supernatants) and represent acrosomal content (A1-A10 in Table S1) while samples P1-P10 are pellets and represent arcosome reacted sperm (S1-S10 in Table S1).
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:55:37.777.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPavel Stopka
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-02 01:16:33ID requested
12023-02-27 07:19:27announced
22023-02-28 06:35:14announced2023-02-28: Updated project metadata.
32023-11-14 07:55:38announced2023-11-14: Updated project metadata.
Publication List
Ot, č, en, á, š, kov, á T, Mac, í, č, kov, á E, Vondr, á, kov, á J, Frol, í, kov, á M, Komrskova K, Stopkov, á R, Stopka P, Proteomic analysis of the mouse sperm acrosome - towards an understanding of an organelle with diverse functionality. Eur J Cell Biol, 102(2):151296(2023) [pubmed]
Keyword List
submitter keyword: Mus musculus,acrosome, label free
Contact List
Pavel Stopka
contact affiliationBIOCEV group, Department of Zoology, Faculty of Science, Charles University in Prague, Průmyslová 595, 252 42 Vestec
contact emailpavel.stopka@natur.cuni.cz
lab head
Pavel Stopka
contact affiliationDepartment of Zoology, Charles University, Prague
contact emailpstopka@natur.cuni.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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