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PXD039127

PXD039127 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMapping the cytosolic proteins of HEK293T cells
DescriptionN-terminal proteoforms stem from the same gene but differ at their N-terminus, and most of these are found to be truncated, though some are N-terminally extended caused by ribosomes starting translation from codons in the annotated 5’UTR, and/or carry modified N-termini different from those of the canonical protein. Biological functions of N-terminal proteoforms are emerging, however, it remains unknown to what extend N-terminal proteoforms further expand the functional complexity. To address this in a more global manner, we mapped the interactomes of several pairs of N-terminal proteoforms and their canonical counterparts. For this, we first generated an in-depth catalogue of N-terminal proteoforms in the cytosol of HEK293T cells. In order to aid the selection of pairs of N-terminal proteoforms we generated a cytosolic map to verify their cytosolic interactions. This localization is crucial as Virotrap will be used for interactome analysis and this method works best for cytosolic proteins.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:47:16.909.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnnelies Bogaert
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-02 01:14:32ID requested
12023-06-08 01:30:55announced
22023-11-14 09:01:07announced2023-11-14: Updated project metadata.
32024-10-22 05:47:17announced2024-10-22: Updated project metadata.
Publication List
10.26508/LSA.20230197;
Keyword List
submitter keyword: cytosol,Fractionation, HEK293T cells, LC-MS/MS
Contact List
Kris Gevaert
contact affiliationVIB Center for Medical Biotechnology, VIB, Ghent, B9052, Belgium Department of Biomolecular Medicine, Ghent University, Ghent, B9052, Belgium
contact emailkris.gevaert@vib-ugent.be
lab head
Annelies Bogaert
contact affiliationVIB - Center for medical Biotechnology Ghent University - Department of Biomolecular Medicine
contact emailAnnelies.bogaert@vib-ugent.be
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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