PXD039121

PXD039121 is an original dataset announced via ProteomeXchange.

Title	L-lactate induced proteomic changes in rat hippocampus
Description	Rats were anesthetized with sodium pentobarbital (50 mg/kg, i.p.) and placed in a stereotaxic instrument (Kopf instrument). After exposing the skull, bilateral craniotomy (0.5 - 0.8 mm holes, 4 mm posterior to bregma and 2.6 mm lateral from midline) was done. In one group of rats (Lactate group), each hippocampus (3.5 mm ventral from the surface of the skull at the craniotomy site) was infused with 100 nmol of L-lactate dissolved in 1µl of ACSF at a flow rate of 0.1 µl/min (controlled by microinjection pump; World Precision Instruments, USA). In another group of rats (ACSF group), 1 µl of artificial cerebrospinal fluid (ACSF; NaCl 124 mM, KCl 3 mM, CaCl2 2.4 mM, MgSO4 1.3 mM, glucose 10 mM, and HEPES 10 mM, pH = 7.3) was infused per HPC. Needle was kept at injection site for additional 5 minutes for the proper dispersion of solution after L-lactate/ACSF infusion. Then rats were kept into their cages for approximately 60 minutes. After that higher dose of sodium pentobarbital was given, and decapitation was done quickly. Following decapitation, the dura mater was removed carefully, and the brain was flipped out from skull into ice cold PBS using spatula. The right and left hippocampus were isolated on an ice-cold plate using sterile forceps and were stored immediately at -80°C until further use. Later, the right hippocampus was used as sample for mass spectrometry (MS).
HostingRepository	jPOST
AnnounceDate	2023-02-23
AnnouncementXML	Submission_2023-02-22_20:58:47.665.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mastura Akter
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-30 21:09:37	ID requested
⏵ 1	2023-02-22 20:58:48	announced

Dataset with its publication pending

submitter keyword: lactate, hippocampus, proteomics, rat

Professor Ying Li
lab head
Mastura Akter
contact affiliation	City University of Hong Kong
dataset submitter

jPOST dataset URI

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