PXD039104
PXD039104 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Peste des petits ruminants virus nucleocapsid protein interacts with PACT and induces stress granule to promote viral replication |
| Description | Stress granules (SGs) formation is a conserved cellular strategy to reduce stress-related damage regulating cell survival. A Mass spectrometry-based profiling of the interactome of PPRV N protein revealed that PPRV N interacted with PACT to regulate the assembly of SGs. N protein inhibited the interaction between PACT and a PKR inhibitor TRBP through binding to the M1 domain of PACT, which enhanced the interaction between PACT and PKR and thus promoted PKR activation and subsequent eIF2α phosphorylation as well as SGs formation. The regulatory function of N protein was strikingly abrogated in PACT-/- cells. PPRV infection-induced SGs through the PKR/eIF2α pathway are PACT-dependent. The loss of function assay indicated that PPRV-induced SGs were critical for PPRV replication. We concluded that the PPRV N protein manipulates the host PKR/eIF2α/SGs axis to favor virus replication. |
| HostingRepository | iProX |
| AnnounceDate | 2022-12-27 |
| AnnouncementXML | Submission_2023-08-14_00:37:38.969.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shuying Chen |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-12-26 19:23:05 | ID requested | |
| 1 | 2022-12-26 19:23:25 | announced | |
| ⏵ 2 | 2023-08-14 00:37:39 | announced | 2023-08-14: Update publication information. |
Publication List
| Chen S, Yang F, Cao W, Zhang X, Liu H, Zhang K, Liu X, Wang J, Zheng H, Zhu Z, Peste Des Petits Ruminants Virus Nucleocapsid Protein Interacts with Protein Kinase R-Activating Protein and Induces Stress Granules To Promote Viral Replication. J Virol, 97(2):e0171222(2023) [pubmed] |
Keyword List
| submitter keyword: PPRV, nucleocapsid protein, PACT, PKR, stress granule |
Contact List
| Zixiang Zhu | |
|---|---|
| contact affiliation | Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences |
| contact email | zhuzixiang@caas.cn |
| lab head | |
| Shuying Chen | |
| contact affiliation | Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences |
| contact email | 745549555@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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