PXD039077

PXD039077 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the extracellular matrix of human atherosclerotic plaques shows marked changes between plaque types
Description	Cardiovascular disease (CVD) is the leading cause of death, with atherosclerosis a major underlying cause. While often asymptomatic for decades, plaque destabilisation and rupture can arise suddenly and cause acute arterial occlusion or peripheral embolisation resulting in acute myocardial infarction, stroke and lower limb ischaemia. Hard plaques are typically considered as stable, and soft plaques as unstable. Extracellular matrix (ECM) remodelling can result in plaque destabilisation, but the mechanisms that drive the development of unstable lipid-rich plaques with a thin fibrous cap, versus stable fibrotic plaques with a thick cap, are not fully understood. We hypothesised that there would be significant differences in ECM composition between hard (stable) and soft (unstable and rupture-prone) plaques. We identified and quantified >46700 proteins, including 367 ECM proteins, with unprecedented coverage and high reproducibility. We identified 575 proteins with differential abundances between hard (stable) and soft (unstable) plaques. Proteins involved in inflammation and ECM remodeling, including multiple proteases were enriched, and ECM proteins decreased, in soft plaques. These data provide a unique insight into inflammatory mechanisms and ECM remodelling as an explanation for plaque destabilization. Furthermore they provide a first step towards identifying circulating biomarkers for individualised risk profiling of arteriosclerosis.
HostingRepository	PRIDE
AnnounceDate	2024-01-15
AnnouncementXML	Submission_2024-01-15_02:00:17.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lasse Gøbel Lorentzen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-22 03:04:18	ID requested
⏵ 1	2024-01-15 02:00:17	announced

10.1016/J.MBPLUS.2024.100141;

submitter keyword: stroke,Cardiovascular disease, atherosclerosis, extracellular matrix

Michael J. Davies
contact affiliation	Department of Biomedical Sciences, Panum Institute, University of Copenhagen, Denmark
contact email	davies@sund.ku.dk
lab head
Lasse Gøbel Lorentzen
contact affiliation	University of Copenhagen
contact email	lassegl@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD039077

PRIDE project URI

• PRIDE
  1. PXD039077
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the extracellular matrix of human atherosclerotic plaques shows marked changes between plaque types