PXD039074 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome analysis of male accessory gland secretions in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) |
Description | In insects, male accessory gland proteins (ACPs) are important reproductive proteins secreted by male accessory glands (MAGs) of the internal male reproductive system. During mating, ACPs were transferred along with sperms inside female bodies and have a significant impact on the physiology of female reproduction. Under sexual selection pressures, the ACPs exhibit remarkably rapid and divergent evolution and varies from species to species. The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a major insect pest of cruciferous vegetables worldwide. The reproductive physiology on post-mating state of this species is still largely unknown, which is important for management of this pest. In this study, the ACPs transferred into females during mating were identified by using a tandem mass tags quantitative proteomic analysis. The MAGs were compared before and after mating immediately. The proteomes of copulatory bursas (CB) in mated females shortly after mating were also analyzed by shotgun LC-MS/MS technique. In total, we identified 123 putative secreted ACPs, including most important physiological: regulators of proteolysis, transporters and protein export machinery, signal transduction and immunity. Comparing P. xylostella with other four insect ACPs, trypsins were the only ACPs detected in all insect species. We also identified some new insect ACPs, including protein with chitin binding Peritrophin-A domain, PMP-22/EMP/MP20/Claudin tight junction domain-containing protein, netrin-1, type II inositol 1,4,5-trisphosphate 5-phosphatase, two spaetzles, allatostatin-CC and cuticular protein. This was the first time to identify and analyze ACPs in P. xylostella. Our results have provided an important list of putative secreted ACPs, and have set the stage for further explore functions of these putative proteins in P. xylostella reproduction. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:43:36.441.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lijuan Wu |
SpeciesList | scientific name: Plutella xylostella (Diamondback moth) (Plutella maculipennis); NCBI TaxID: 51655; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-22 03:00:02 | ID requested | |
1 | 2023-03-11 10:52:17 | announced | |
⏵ 2 | 2023-11-14 08:43:45 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wu LJ, Li F, Song Y, Zhang ZF, Fan YL, Liu TX, (Lepidoptera: Plutellidae). Insects, 14(2):(2023) [pubmed] |
Keyword List
submitter keyword: Plutella xylostella |
seminal fluid proteins |
male accessory gland |
accessory gland proteins |
proteomics |
Contact List
Lijuan Wu |
contact affiliation | College of Plant Protection, Northwest A&F University |
contact email | wu_lijuan@nwafu.edu.cn |
lab head | |
Lijuan Wu |
contact affiliation | College of Plant Protection, Northwest A&F University |
contact email | wu_lijuan@nwafu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039074
- Label: PRIDE project
- Name: Proteome analysis of male accessory gland secretions in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)