PXD039063

PXD039063 is an original dataset announced via ProteomeXchange.

Title	Identification of CD24 interaction partners in urologic tumor cell lines
Description	Being involved in adhesion, migration, and invasion, the highly glycosylated signal transducer CD24 (cluster of differentiation 24) has been implicated to play an essential role during carcinogenesis. Previously, the molecular and (epi)genetic regulation of CD24 has been characterized in testicular germ cell tumors (GCT). Here, CD24 was exclusively found in embryonal carcinoma (EC), which represents the stem cell like population of GCT (see project PXD025110). For a better understanding of the molecular function of CD24, this study aimed at the identification of the direct interaction partners of CD24 not only in GCTs, but also in other urologic malignancies, such as urothelial- (UC), prostate- (PC), and renal cell carcinoma (RCC). For this purpose, co-immunoprecipitations of CD24 were performed in GCT, UC, PC, and RCC cell lines, while CD24-deficient EC cells as well as IgG2a controls were included for high validity. Extracted proteome was measured by liquid-chromatography coupled with mass spectrometry (LS-MS).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:02.788.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anja Stefanski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-22 02:25:45	ID requested
1	2023-05-10 17:33:37	announced
⏵ 2	2023-11-14 08:48:07	announced	2023-11-14: Updated project metadata.

Skowron MA, Kotthoff M, Bremmer F, Ruhnke K, Parmaksiz F, Richter A, K, ü, ffer S, Reuter-Jessen K, Pauls S, Stefanski A, Str, ö, bel P, St, ü, hler K, Nettersheim D, an antibody-drug-conjugate and studying therapy resistance of yolk-sac tumors to identify and screen specific therapeutic options. Mol Med, 29(1):40(2023) [pubmed]

submitter keyword: Germ cell tumor, CD24, urothelial carcinoma, renal cell carcinoma, co-immunoprecipitation, prostate cancer

Kai Stühler
contact affiliation	Molecular Proteomics Laboratory, Heinrich Heine University Düsseldorf, Düsseldorf, GermanyMolecular Proteomics Laboratory, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
contact email	kai.stuehler@hhu.de
lab head
Anja Stefanski
contact affiliation	Molecular Proteomics Laboratory
contact email	anja.stefanski@hhu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039063

PRIDE project URI

• PRIDE
  1. PXD039063
     1. Label: PRIDE project
     2. Name: Identification of CD24 interaction partners in urologic tumor cell lines