The number of patients infected with simian malaria is increasing in many countries in Southeast Asia. The behavior of humans, monkeys, and vectors influences their interactions with each other and is the most important risk factor of zoonotic malaria infection. However, no serum proteomics study has been conducted in wild macaques. The present study was performed using a proteomics approach to explore the protein expression profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites.