PXD039044

PXD039044 is an original dataset announced via ProteomeXchange.

Title	Large-scale proteomic analysis of T. spiralis muscle-stage ESPs identifies a novel motif for prediction of secreted products
Description	The Trichinella genus contains parasitic nematodes capable of infecting a wide range of hosts including mammals, birds and reptiles. Like other helminths, T. spiralis secretes a complex mixture of bioactive molecules capable of modulating its immediate surroundings and creating a hospitable environment for growth, survival and ultimately transmission. The constitution of these excretory-secretory products (ESPs) changes depending on the tissue niche and the specific stage of parasite development. Unique to T. spiralis is a true intracellular stage wherein larvae develop inside striated myotubes. Remarkably, the parasite larvae do not destroy the host cell but rather reprogram it to support their presence and growth. This transformation is largely mediated through stage-specific secretions released into the host cell cytoplasm. In this study, we apply state of the art proteomics and computational approaches to elucidate the composition and functions of muscle-stage T. spiralis ESPs. Moreover, we define a recurring, upstream motif associated with the stichosome, the main secretory organ of this worm, and can be used to predict secreted proteins across experimentally less tractable T. spiralis life cycle stages.
HostingRepository	iProX
AnnounceDate	2022-12-21
AnnouncementXML	Submission_2022-12-21_16:53:21.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Weekes
SpeciesList	scientific name: Trichinella spiralis; NCBI TaxID: 6334; scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	methionine oxidation with neutral loss of 64 Da
Instrument	LTQ Orbitrap; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-21 16:53:09	ID requested
⏵ 1	2022-12-21 16:53:21	announced

Dataset with its publication pending

submitter keyword: Trichinella, proteomic, muscle, excretory-secretory products

Michael Weekes
contact affiliation	University of Cambridge
contact email	mpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliation	University of Cambridge
contact email	mpw1001@cam.ac.uk
dataset submitter

iProX dataset URI