PXD039043

PXD039043 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of Nrg1 enhancer binding proteins
Description	Hyperglycemia is a risk factor for breast cancer-related morbidity and mortality. Hyperglycemia induces Neuregulin 1 (Nrg1) overexpression in breast cancer, which subsequently promotes tumor progression. However, molecular mechanisms underlying hyperglycemia-induced Nrg1 overexpression remain poorly understood. Here, We used DNA-protein pull-down using Nrg1 enhancer sequence as bait followed by LC/MS and identified RBPJ as a key component of the Nrg1 enhanceosome. We show that hyperglycemia causes active histone modifications at the Nrg1 enhancer, forming enhanceosome complexes where RBPJ, P300, and SETD1A are recruited to upregulate Nrg1 expression.
HostingRepository	MassIVE
AnnounceDate	2022-12-21
AnnouncementXML	Submission_2022-12-21_17:12:10.154.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	changhu lee
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-21 16:24:54	ID requested
⏵ 1	2022-12-21 17:12:10	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Breast cancer cell, HIgh glucose, Nrg1 enhanceosome

submitter keyword: Breast cancer cell, HIgh glucose, Nrg1 enhanceosome

Contact List

Jiyoung Park
contact affiliation	UNIST
contact email	jpark@unist.ac.kr
lab head
changhu lee
contact affiliation	UNIST
contact email	hulee@unist.ac.kr
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090951/