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PXD039040

PXD039040 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhototropin2 3'UTR overlaps with the AT5G58150 gene encoding an inactive RLK kinase
DescriptionThis study aimed to investigate the biological implications of two overlapping sequences in the Arabidopsis genome, the 3UTR of the PHOT2 gene and a putative AT5G58150 gene, encoded on the complementary strand. AT5G58150 is a probably inactive protein kinase that belongs to the transmembrane, leucine rich repeat receptor like kinase family. Phot2 is a UV/blue light, membrane-bound photoreceptor kinase. Thus, both proteins share their cellular localization, apart from the loci proximity. Both the at5g58150 T-DNA SALK_093781C and 35S::AT5G58150-GFP lines overexpress the AT5G58150 gene. However, a detailed analysis did not prove any substantial impact of PHOT2 or AT5G58150 on their mutual expression levels in the different light and osmotic stress conditions. This may result from only a small overlap found for 3UTR regions of both genes consisting of 66 bp. AT5G58150 is a plasma membrane protein, with no apparent kinase activity. We could not observe AT5G58150 homodimer formation or any significant interaction with PHOT2. Our findings show that AT5G58150 may be involved in the development of root structures such as primordia or lateral roots. In line with this, mass spectrometry analysis identified proteins with ATPase activity, which are involved in proton transport and cell elongation, as putative interactors of AT5G58150. Membrane kinases, including other members of the LRR RLK family and BSK kinases (positive regulators of brassinosteroid signalling), can also act as partners for AT5G58150.
HostingRepositoryMassIVE
AnnounceDate2023-03-20
AnnouncementXMLSubmission_2023-03-20_01:00:06.891.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterUrszula Jankowska
SpeciesList scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-21 08:42:29ID requested
12023-03-20 01:00:07announced
Publication List
no publication
Keyword List
submitter keyword: Arabidopsis, AT5G58150, LRR RLK, kinase, phototropin2
Contact List
Justyna Labuz
contact affiliationMalopolska Centre of Biotechnology, Jagiellonian University
contact emailjustyna.sojka@uj.edu.pl
lab head
Urszula Jankowska
contact affiliationJagiellonian University
contact emailurszula.jankowska@uj.edu.pl
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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