PXD039035

PXD039035 is an original dataset announced via ProteomeXchange.

Title	Multiplexed quantitative proteomics of CD4 T cell-mediated postsynaptic dendritic cells (DC)
Description	We aimed to characterize the proteome remodeling in DCs induced upon antigen presentation. To do so, we used an in vitro antigen presentation model to generate postsynaptic (psDC) or nonsynaptic (nsDC) DCs similarly to previously reported (Alcaraz-Serna et al., 2021). Bone marrow-derived DCs (BMDCs), which had been activated with LPS and pulsed (psDC) or not (nsDC) with the ovalbumin peptide OVA323-339, were co-cultured with resting CD4+ T cells from OT-II mice to allow cognate antigen-dependent immune synapse formation (psDC) or not (nsDC). DCs were then purified for proteomic analysis by using mass spectrometry based on multiplexed isotopic labeling peptides approach.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:22:28.834.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inmaculada Jorge
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; dihydroxylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-21 06:43:55	ID requested
⏵ 1	2024-01-26 07:22:29	announced

Calzada-Fraile D, Iborra S, Ram, í, rez-Huesca M, Jorge I, Dotta E, Hern, á, ndez-Garc, í, a E, Mart, í, n-C, ó, freces N, Nistal-Vill, á, n E, Veiga E, V, á, zquez J, Pasqual G, S, á, nchez-Madrid F, T cells. Nat Commun, 14(1):6772(2023) [pubmed]

10.1038/s41467-023-42480-3;

submitter keyword: mouse, CD4 T cell-mediated postsynaptic dendritic cells (DC) , quantitative proteomics, LC-MS/MS

Jesús Vázquez Cobos
contact affiliation	Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid (Spain)
contact email	jvazquez@cnic.es
lab head
Inmaculada Jorge
contact affiliation	Vascular Biology and Inflammation
contact email	ijorge@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD039035

PRIDE project URI

• PRIDE
  1. PXD039035
     1. Label: PRIDE project
     2. Name: Multiplexed quantitative proteomics of CD4 T cell-mediated postsynaptic dendritic cells (DC)