PXD039015

PXD039015 is an original dataset announced via ProteomeXchange.

Title	Whole cell proteimics of leptotene+zygotene(LZ) and pachytene+diplotene+diakinesis (PDD) of first meiosis prophase of spermatogenesis in male mouse.
Description	2-hydroxyglutarate (2HG) is recognized as an epigenetic regulator in cancer and some transient biological processes. Of all organs, the testis harbors the highest baseline physiological levels of 2HG, yet it’s putative functions in germ cell biology are unknown. Here we show that 2HG is generated in specific stages of spermatogenesis by the testis specific lactate dehydrogenase C (LDHC), beginning at the last stages of prophase I. Unexpectedly LDHC enters nuclei and concentrates in centromeres. LDHC-generated L-2HG controls centromere condensation and pericentromeric heterochromatin organization through multiple effects including clustering of chromocenters, centromere and chromocenter condensation and expression of satellite RNAs. These effects are rapid and specific to L but not D-2HG. In vivo depletion of L-2HG causes centromere malfunction and activation of the spindle assembly checkpoint. Our findings reveal that 2HG can directly affect centromere and pericentromeric heterochromatin conformation and function and is necessary for licensing chromosome segregation.
HostingRepository	PRIDE
AnnounceDate	2025-05-23
AnnouncementXML	Submission_2025-05-23_04:48:59.696.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eli Pikarsky
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-21 00:02:57	ID requested
⏵ 1	2025-05-23 04:49:00	announced

Dataset with its publication pending

submitter keyword: Testis, Meiosis, Spermatogenesis, LC-MS/MS

Eli Pikarsky
contact affiliation	The Concern Foundation Laboratories at The Lautenberg Center for Immunology and Cancer Research IMRIC, Faculty of Medicine, The Hebrew University, Jerusalem, Israel
contact email	Peli@hadassah.org.il
lab head
Eli Pikarsky
contact affiliation	The Concern Foundation Laboratories at The Lautenberg Center for Immunology and Cancer Research IMRIC, Faculty of Medicine, The Hebrew University, Jerusalem, Israel
contact email	Peli@hadassah.org.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD039015

PRIDE project URI

• PRIDE
  1. PXD039015
     1. Label: PRIDE project
     2. Name: Whole cell proteimics of leptotene+zygotene(LZ) and pachytene+diplotene+diakinesis (PDD) of first meiosis prophase of spermatogenesis in male mouse.